Effect of mitophagy related genes on the antioxidant properties of <i>Saccharomyces cerevisiae</i>.

Wanqi Cheng; Qianyao Hou; Chunfeng Liu; Chengtuo Niu; Feiyun Zheng; Qi LI; Jinjing Wang

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Effect of mitophagy related genes on the antioxidant properties of Saccharomyces cerevisiae.

Author: Wanqi CHENG ¹ ; Qianyao HOU ¹ ; Chunfeng LIU ¹ ; Chengtuo NIU ¹ ; Feiyun ZHENG ¹ ; Qi LI ¹ ; Jinjing WANG ¹
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1. Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
Publication Type:Journal Article
Keywords: ATG genes; antioxidant properties; mitophagy; oxidative stress
MeSH: Mitophagy/genetics*; Saccharomyces cerevisiae/genetics*; Antioxidants; Hydrogen Peroxide/pharmacology*; Reactive Oxygen Species
From: Chinese Journal of Biotechnology 2023;39(8):3464-3480
CountryChina
Language:Chinese
Abstract: Mitophagy is a process whereby cells selectively remove mitochondria through the mechanism of autophagy, which plays an important role in maintaining cellular homeostasis. In order to explore the effect of mitophagy genes on the antioxidant activities of Saccharomyces cerevisiae, mutants with deletion or overexpression of mitophagy genes ATG8, ATG11 and ATG32 were constructed respectively. The results indicated that overexpression of ATG8 and ATG11 genes significantly reduced the intracellular reactive oxygen species (ROS) content upon H₂O₂ stress for 6 h, which were 61.23% and 46.35% of the initial state, respectively. Notable, overexpression of ATG8 and ATG11 genes significantly increased the mitochondrial membrane potential (MMP) and ATP content, which were helpful to improve the antioxidant activities of the strains. On the other hand, deletion of ATG8, ATG11 and ATG32 caused mitochondrial damage and significantly decreased cell vitality, and caused the imbalance of intracellular ROS. The intracellular ROS content significantly increased to 174.27%, 128.68%, 200.92% of the initial state, respectively, upon H₂O₂ stress for 6 h. The results showed that ATG8, ATG11 and ATG32 might be potential targets for regulating the antioxidant properties of yeast, providing a new clue for further research.