The Uptake and Distribution Evidence of Nano- and Microplastics <i>in vivo</i> after a Single High Dose of Oral Exposure.

Tao Hong; Wei Sun; Yuan Deng; Jian Da Lyu; Cui Hong Jin; Ying Long Bai; Jun NA; Rui Zhang; Yuan Gao; Guo Wei Pan; Zuo Sen Yang; Ling Jun Yan

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The Uptake and Distribution Evidence of Nano- and Microplastics in vivo after a Single High Dose of Oral Exposure.

Author: Tao HONG ^{1
,

2} ; Wei SUN ³ ; Yuan DENG ³ ; Jian Da LYU ³ ; Cui Hong JIN ⁴ ; Ying Long BAI ⁵ ; Jun NA ^{6
,

7} ; Rui ZHANG ^{6
,

7} ; Yuan GAO ³ ; Guo Wei PAN ³ ; Zuo Sen YANG ^{6
,

7} ; Ling Jun YAN ³
Author Information

1. Research Center for Universal Health, School of Public Health, China Medical University, Shenyang 110122, Liaoning, China
2. Department of Pain Management, Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning, China.
3. Research Center for Universal Health, School of Public Health, China Medical University, Shenyang 110122, Liaoning, China.
4. Department of Toxicology, School of Public Health, China Medical University, Shenyang 110122, Liaoning, China.
5. Department of Maternal and Child Health, School of Public Health, China Medical University, Shenyang 110122, Liaoning, China.
6. Institute of Preventive Medicine, China Medical University, Shenyang 110122, Liaoning, China
7. Institute of Chronic Diseases, Liaoning Provincial Center for Disease Control and Prevention, Shenyang 110000, Liaoning, China.
Publication Type:Journal Article
Keywords: Blood; IVIS Spectrum small-animal imaging system; Microplastics; Nanoplastics; Tissue distribution
MeSH: Microplastics; Plastics; Liver; Microspheres; Lung; Water Pollutants, Chemical
From: Biomedical and Environmental Sciences 2024;37(1):31-41
CountryChina
Language:English
Abstract: OBJECTIVE:Tissue uptake and distribution of nano-/microplastics was studied at a single high dose by gavage in vivo.
METHODS:Fluorescent microspheres (100 nm, 3 μm, and 10 μm) were given once at a dose of 200 mg/(kg∙body weight). The fluorescence intensity (FI) in observed organs was measured using the IVIS Spectrum at 0.5, 1, 2, and 4 h after administration. Histopathology was performed to corroborate these findings.
RESULTS:In the 100 nm group, the FI of the stomach and small intestine were highest at 0.5 h, and the FI of the large intestine, excrement, lung, kidney, liver, and skeletal muscles were highest at 4 h compared with the control group ( P < 0.05). In the 3 μm group, the FI only increased in the lung at 2 h ( P < 0.05). In the 10 μm group, the FI increased in the large intestine and excrement at 2 h, and in the kidney at 4 h ( P < 0.05). The presence of nano-/microplastics in tissues was further verified by histopathology. The peak time of nanoplastic absorption in blood was confirmed.
CONCLUSION:Nanoplastics translocated rapidly to observed organs/tissues through blood circulation; however, only small amounts of MPs could penetrate the organs.