Up-regulation of Proinflammatory Cytokines in Rostral Ventromedial Medulla Contributes to Chronic Postsurgical Pain by Promoting 5-HT Release
10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).20240004.005
- VernacularTitle:RVM致炎细胞因子上调导致5-HT释放参与调控慢性术后疼痛
- Author:
Juanli DAI
1
;
Zhen WANG
1
;
Chaoxiong DONG
1
;
Yuying LI
2
;
Xuhong WEI
3
Author Information
1. Xiehe Shenzhen Hospital， Huazhong University of Science and Technology， Shenzhen 518052， China
2. Shenzhen Polytechnic University， Shenzhen 518055， China
3. Department of Physiology， Zhongshan School of Medicine // Department of Physiology and Pain Research Center， Sun Yat-Sen University， Guangzhou 510080， China
- Publication Type:Journal Article
- Keywords:
chronic postoperative pain （CPSP）;
rostral ventromedial medulla （RVM）;
tumor necrosis factor alpha （TNFα）;
interleukin-1β （IL-1β）;
5-Hydroxytryptamine （5-HT）
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2024;45(1):54-62
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the role of proinflammatory cytokines tumor necrosis factor alpha （TNFα） and interleukin-1β （IL-1β） in rostral ventromedial medulla （RVM） in chronic postsurgical pain （CPSP） induced by skin/muscle incision and retraction （SMIR）. MethodsSD rats were randomly divided into 5 groups： ① Sham group； ② SMIR group； ③ SMIR+TNFα/IL-1β neutralizing antibody group； ④ SMIR+TNFα/IL-1β group and ⑤ SMIR+vehicle group. 50% paw mechanical withdrawal threshold （MWT） was measured by the up-down method， immunofluroscence was used to detect the TNFα and IL-1β expression and ELISA for the 5-Hydroxytryptamine （5-HT） level. ResultsSMIR elicited persistent nociceptive sensitization， upregulated TNFα and IL-1β expression in RVM neurons and astrocytes. Microinjection of TNFα or IL-1β neutralizing antibody into RVM inhibited the development of nociceptive sensitization and decreased the level of 5-HT in both RVM and spinal dorsal horn. While microinjection of recombinant TNFα or IL-1β into RVM enhanced the development of nociceptive sensitization and increased the level of 5-HT in both RVM and spinal dorsal horn. ConclusionUp-regulation of proinflammatory cytokines in RVM may contribute to SMIR induced CPSP by promoting 5-HT release.
