Acaricidal, larvacidal, and repellent activity of green synthesized silver nanoparticles against Hyalomma dromedarii
https://doi.org/10.47665/tb.40.3.013
- Author:
Majeed, Q.A.H.
1
;
Gattan, H.
2
;
Al-Ahmadi, B.M.
3
;
Shater, A.F.
4
;
Alanazi, A.D.
5
;
Alazemi, M.S.H.
1
Author Information
1. Department of Science, College of Basic Education, PAAET, Post code 23167, Aridiya, Kuwait
2. Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia, Special Infectious Agents Unit, King Fahad Medical Research Center, Jeddah, Saudi Arabia
3. Department of Biology, Faculty of Science, Taibah University, Taibah, Saudi Arabia
4. Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk 71491, Saudi Arabia
5. Department of Biological Sciences, Faculty of Science and Humanities, Shaqra University, P.O. Box 1040, Ad-Dawadimi 11911, Saudi Arabia
- Publication Type:Journal Article
- Keywords:
Insecticide;
nanoparticles;
tick;
larvae;
control.
- From:Tropical Biomedicine
2023;40(No.3):356-362
- CountryMalaysia
- Language:English
-
Abstract:
We aimed at determination of acaricidal, larvacidal, and repellent activities of green synthesized
silver nanoparticles (SNP) against Hyalomma dromedarii as one of the most common ticks in camels.
SNP were green synthesized by reducing Lupinus albus extract through the precipitation technique.
The acaricidal, larvicidal, and repellent activity of SNP against H. dromedarii was studied through the
adult immersion test (AIT), the larval packet test (LPT), the vertical movement behavior of tick’s larvae
method, anti-acetylcholinesterase (AChE) activity, and oxidative enzyme activity. The green synthesized
SNP displayed a spherical form with a size ranging from 25–90 nm; whereas the most distribution of
particles size was reported at 50-65 nm. SNP dose-dependently (p<0.001) increased the mortality rate
of H. dromedarii adult; whereas at 16 and 32 µg/mL completely killed the adult females. Treatment of
exposure of H. dromedarii adult to SNP markedly (p<0.001) declined the mean number, weight, and
hatchability of eggs. Treatment of H. dromedarii larvae with SNP reduced the viability rate of larvae
with the LC50 and LC90 values of 3.1 and 6.9 µg/mL, respectively. Exposure of H. dromedarii larvae to
SNP, especially at ½ LC50 and LC50, markedly (p<0.001) increased the oxidative stress and declined the
level of antioxidant enzymes in H. dromedarii larvae; whereas, markedly suppressed the AChE activity
of the larvae stage of H. dromedarii in comparison to the control group. These results showed that SNP
green synthesized by L. albus extract had promising acaricidal, larvicidal and repellent activity against
H. dromedarii adults and larvae as a dose-dependent response. SNP also considrably decreased the
level of acetylcholinesterase and antioxidant activity and also provokes oxidative stress in H. dromedarii
larvae. However, more investigation must be designed to clear the accurate mechanisms and the efficacy
of SNP in practical use.
- Full text:8.2023my1429.pdf
