Structural stability of Hib capsular polysaccharide in vaccine manufacturing
10.13200/j.cnki.cjb.004167
- VernacularTitle:Hib荚膜多糖在疫苗制备周期中的结构稳定性
- Author:
ZHANG Tengteng
- Publication Type:Journal Article
- Keywords:
Haemophilus influenzaetype b(Hib);
Capsular polysaccharide;
Polyribosylribitol phosphate(PRP);
Nuclear magnetic resonance spectroscopy(NMR);
Structure;
Stability
- From:
Chinese Journal of Biologicals
2024;37(2):129-137
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the stability of polyribosylribitol phosphate(PRP),the basic structure of capsular polysaccharide of Haemophilus influenzae type b(Hib),in the preparation of Hib conjugate vaccine.Methods The structures of the prepared Hib polysaccharides,polysaccharide derivatives and protein-conjugated polysaccharides were analyzed by nuclear magnetic resonance spectroscopy(NMR).Results The detection results of the prepared Hib polysaccharides,polysaccharide derivatives and protein-conjugated polysaccharides all met the requirements of relevant standards of Chinese Pharmacopoeia(VolumeⅢ,2020 edition),and the NMR spectra showed no significant change.Conclusion The basic structure PRP of the main carbohydrate antigen of Hib conjugate vaccine had no change during the vaccine manufacturing.
