Simultaneous determination of three constituents in Chunyang Zhengqi capsules by HPLC
10.12206/j.issn.2097-2024.202201059
- VernacularTitle:高效液相色谱法同时测定纯阳正气胶囊中3种成分的含量
- Author:
Yi WEN
1
;
Lulu MENG
2
;
Baoyi WANG
1
;
Feixue WEI
1
;
Peng DENG
1
;
Rongying LIU
3
Author Information
1. Eight Plus One Pharmaceutical Co., Ltd, Guilin 541000, China.
2. Guilin Food and Drug Control Institute, Guilin 541012, China.
3. School of Pharmacy, Guilin Medical College, Guilin 541000, China.
- Keywords:
Chunyang Zhengqi capsules;
hesperidin;
cinnamaldehyde;
eugenol;
content determination
- From:
Journal of Pharmaceutical Practice and Service
2024;42(2):78-81
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish method for simultaneous determination of hesperidin, cinnamaldehyde and eugenol in Chunyang Zhengqi capsules by high performance liquid chromatography. Methods The column was Agilent PorosheⅡ 120 EC-C18 (4.6 mm×150 mm, 4 μm). The mobile phase was acetonitrile-water with gradient elution. The column temperature was 35℃. The flow rate was 1.0 ml/min, and the detection wavelength was 284 nm. Results The methodological verification showed that hesperidin, cinnamaldehyde and eugenol had a good linearity (r≥0.999 9). The precisions were less than 2.0%. The average recovery was between 98.0% and 101.9%. The stability and repeatability of RSD were also less than 3.0%, which met the requirements of method validation. Conclusion The method is simple, stable, reproducible and accurate, which could be used to the quality control of Chunyang Zhengqi capsules.