Development and application of in vitro intestinal absorption model based on microfluidic chips
10.12206/j.issn.2097-2024.202305003
- VernacularTitle:基于微流控芯片的体外肠道吸收模型构建及其应用进展
- Author:
Lan CHEN
1
;
Xiaoli HE
1
;
Piaoxue YOU
1
;
Hui WANG
2
,
3
;
Zhanying HONG
2
,
3
,
4
Author Information
1. School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China.
2. School of Pharmacy, Naval Medical University, Shanghai 200433, China
3. Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Shanghai 200433, China.
4. School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China
- Keywords:
microfluidic chips;
intestinal absorption model;
microenvironment;
applications
- From:
Journal of Pharmaceutical Practice and Service
2024;42(2):43-49
- CountryChina
- Language:Chinese
-
Abstract:
The intestine is the main site of oral drug absorption, and the epithelial cells of the intestine contain villi and microvilli, which promote secretion, cell adhesion, and absorption by increasing surface area and other factors. Traditional two-dimensional/three-dimensional (2D/3D) cell culture models and animal models have played an important role in studying drug absorption, but their application is limited due to the lack of sufficient predictability of human pharmacokinetics or ethical issues, etc. Therefore, mimicking the core structure and key functions of the human intestine based on in vitro live cells has been the focus of research on constructing a microfluidic chip-based intestinal model. The model is a microfluidic chip bionic system that simulates the complex microstructure, microenvironment, and physiological functions of the human intestine using microfabrication technology. Compared with 2D cell culture and animal experiments, the intestinal microarray model can effectively simulate the human in vivo environment and is more specific in drug screening. The research progress and applications in disease modeling, drug absorption and transport of intestinal microarray models and intestine-related multi-organ coupled microarray models at home and abroad were reviewed in this paper. The current challenges of intestinal chip simulating intestinal homeostasis and diseases were summarized，in order to provide reference for the further establishment of a more reliable in vitro intestinal chip model.
