Theileria orientalis Buffeli pathotype in cows in a theileriosis-endemic region of India
https://doi.org/10.47665/tb.40.2.015
- Author:
Sahoo, S.
1
;
Sahoo, N.
1
;
Biswal, S.
1
;
Mohanty, B.N.
2
;
Behera, B.
3
;
Pahari, A.
3
Author Information
1. Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneswar-751003, Odisha, India
2. Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneswar-751003, Odisha, India
3. Centre for Wildlife Health, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneswar-751003, Odisha, India
- Publication Type:Journal Article
- Keywords:
Parasitemia;
major piroplasm surface protein;
hemoprotozoa;
piroplasms.
- From:Tropical Biomedicine
2023;40(No.2):236-240
- CountryMalaysia
- Language:English
-
Abstract:
Oriental theileriosis caused by Theileria orientalis is a growing health concern of lactating cows in its
endemic areas. Rapid and sensitive diagnostic tests are demand areas for appropriate and effective
prophylactic and therapeutic measures. Quantitative polymerase chain reaction (qPCR) is the answer
for both detection and quantification of parasites. Present study deals with qPCR for detection of
parasitemia level of T. orientalis in apparently healthy and clinically affected cows. Major piroplasm
surface protein (MPSP) gene present in T. orientalis was cloned in pUC57 vector and transformed into
E. coli Top 10 cells. Single and mixed infections of hemoprotozoa other than T. orientalis, causing anemia
were differentiated through blood smear examination and PCR tests. T. orientalis was detected in 108
(63.15%) ill and 48 (26.66%) healthy cows. Piroplasms detected per 1000 red blood cells (RBCs) was
0-1 in the healthy group as compared to 3-22 in those showing clinical signs. Parasitemia in ill cows
ranged between 6.9 × 102 and 4.5 × 103 parasites / µl of blood which was significantly higher (p<0.05)
than healthy group (2.6 × 102 - 5.7 × 102 parasites / µl of blood). Phylogenetic study of the isolates
showed similarity with Buffeli type that unfolded its pathogenic form in apparently healthy and ill cows.
- Full text:8.2023my1411.pdf
