Molecular identification and in vitro assessment of zoonotic-potential of a novel Orthobunyavirus isolated from broiler chicken in Malaysia
https://doi.org/10.47665/tb.40.2.011
- Author:
Mohamed, M.
1
;
Azmi, M.A.
1
;
Sani, N.I.
1
;
Gilbert, G.
1
;
Reduan, M.H.F.
1
Author Information
1. Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Pengkalan Chepa, 16100 Kota Bharu, Kelantan, Malaysia
- Publication Type:Journal Article
- Keywords:
Zoonotic-potential;
Novel Orthobunyavirus;
broiler;
Malaysia;
interferon-a.
- From:Tropical Biomedicine
2023;40(No.2):194-198
- CountryMalaysia
- Language:English
-
Abstract:
We have previously isolated a novel avian Orthobunyavirus, Kedah Fatal Kidney Syndrome (KFKS) virus
from a broiler farm in Kedah, Malaysia in 2020 with a severe kidney lesion in chickens. The virus was
designated as KFKS2_CS virus. Sequence analysis of partial nucleocapsid (N) and nonstructural (NSs)
sequence of this virus showed the highest sequence identity with previous KFKS1 from Malaysia
(100%) and 97% with a zoonotic Umbre (UMB) virus, which was reported to cause encephalitis in
immunocompromised humans in India. Phylogenetic analysis revealed that this virus was clustered
together with previous KFKS1 virus from Malaysia, UMB and Cristoli viruses. This study aimed to assess
the zoonotic potential of this KFKS2_CS virus in vitro by determining its ability to inhibit the production
of interferon (IFN) in human glioblastoma multiforme (GBM) brain cells using reverse-transcriptase
polymerase reaction (RT-PCR). This virus blocked the production of interferon-a in this human brain
cells. In conclusion, this KFKS2_CS virus may have a zoonotic potential and become a public health
concern in the future.
- Full text:8.2023my1407.pdf
