Expression and clinical significance of NLRP1 and NLRP3 in colonic tissues of patients with ulcerative colitis

Xiao LI; Xin Liu; Yahua Song; Juhui Zhao; Fenrong Chen; Gang Zhao; Yan Wang; Han LI; Haitao Shi

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Expression and clinical significance of NLRP1 and NLRP3 in colonic tissues of patients with ulcerative colitis

VernacularTitle:NLRP1及NLRP3在溃疡性结肠炎患者结肠组织中的表达及其临床意义
Author: Xiao LI ^{1
,

2
,

3} ; Xin LIU ^{1
,

2
,

3} ; Yahua SONG ^{1
,

2
,

3} ; Juhui ZHAO ^{1
,

2
,

3} ; Fenrong CHEN ^{1
,

2
,

3} ; Gang ZHAO ^{1
,

2
,

3} ; Yan WANG ^{1
,

2
,

3} ; Han LI ^{1
,

2
,

3} ; Haitao SHI ^{1
,

2
,

3}
Author Information

1. Department of Gastroenterology, The Second Affiliated Hospital of Xi’an Jiaotong University
2. Shaanxi Key Laboratory of Gastrointestinal Motility Disorders
3. Shaanxi Clinical Research Center of Gastrointestinal Disease, Xi’an 710004, China
Publication Type:Journal Article
Keywords: ulcerative colitis; inflammasome; NLRP3; NLRP1; CRP; endoscopy
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2021;42(1):75-80,112
CountryChina
Language:Chinese
Abstract: 【Objective】 To investigate the expressions of NLRP1 and NLRP3 in the colon of ulcerative colitis (UC) patients and analyze the correlation of the expressions with severity of UC, endoscopic manifestations and related laboratory indicators. 【Methods】 We collected biopsical specimens obtained with colonoscopy in 46 patients with UC (22 mild cases and 24 moderate to severe cases) and 20 cases of normal control group. We used the disease activity index to evaluate the Mayo UC inflammatory activity and immunohistochemical method to detect the protein expression levels of intestinal mucosal NLRP1 and NLRP3 in the tissue. RT-PCR was used to detect the expressions of NLRP1 and NLRP3 mRNA in intestinal mucosal tissues. Meanwhile, the colonoscopy, serum uric acid, C-reactive protein, serum sedimentation rate, platelet count, low-density lipoprotein, and cholesterol of UC patients were also counted to further analyze the relationship between NLRP1 and NLRP3. 【Results】 The expressions of NLRP1 and NLRP3 protein and mRNA in colonic mucosal tissues of UC patients were significantly higher than those of normal controls (P<0.05). Compared with that in mild UC, the expression of NLRP1 in colonic mucosal tissues of moderate and severe UC patients was significantly increased (P<0.05). There was no significant difference in the expressions of NLRP1 and NLRP3 in colonic mucosal tissues of UC patients with different lesion ranges. NLRP1 expression was positively correlated with Mayo overall score, Mayo endoscopic score, erythrocyte sedimentation rate, and C-reactive protein (P<0.05), NLRP3 expression was positively correlated with C-reactive protein (P<0.05), but not correlated with Mayo overall score, Mayo endoscopic score, or erythrocyte sedimentation rate. NLRP1 expression was positively correlated with low-density lipoprotein and platelet (P<0.05), but not with uric acid or cholesterol. NLRP3 was positively correlated with low-density lipoprotein, uric acid and cholesterol (P<0.05), but not with platelet. 【Conclusion】 NLRP1 and NLRP3 may be involved in the pathogenesis of UC and related to disease activity. Therefore, they can be used as molecular targets for targeted therapy, and NLRP1 can be used as a predictor of mucosal healing.