Reverse effect of lentinanin on Sorafenib resistance in cultured hepatocellular carcinoma cells and its mechanism

Lili Han; Fei WU; Lanxuan Huang; Shuqun Zhang

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Reverse effect of lentinanin on Sorafenib resistance in cultured hepatocellular carcinoma cells and its mechanism

VernacularTitle:香菇多糖对培养肝癌细胞索拉非尼耐药的逆转作用及机制
Author: Lili HAN ¹ ; Fei WU ¹ ; Lanxuan HUANG ¹ ; Shuqun ZHANG ¹
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1. Department of Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710004, China
Publication Type:Journal Article
Keywords: lentinan; hepatocellular carcinoma (HCC); Sorafenib; drug resistance; HIF-α; VEGF
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2021;42(1):48-52,64
CountryChina
Language:Chinese
Abstract: 【Objective】 To investigate the effect of lentinan on Sorafenib resistance in hepatocellular carcinoma (HCC) cells BEL-7402/S and its mechanism. 【Methods】 CCK-8 method was used to detect the proliferation-toxicity of lentinan and Sorafenib on BEL-7402 and BEL-7402/S cells, and the drug resistance reversal multiple of Sorafenib. The effects of lentinan on mRNA and protein expressions of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor (HIF-1α) in BEL-7402/S cells were determined by RT-qPCR and Western blotting. 【Results】 Lentinan reduced the proliferation of BEL-7402 and BEL-7402/S cells. Lentinan showed at least a partial reversal of drug resistance to sorafenib in BEL-7402/S cells and down-regulated the mRNA and protein expressions of VEGF and HIF-1α in BEL-7402/S cells. 【Conclusion】 Lentinan can partially reverse the effect of BEL-7402/S cells on Sorafenib resistance, and the mechanism may be related to the down-regulation of VEGF and HIF-1α expressions.