Establishment and identification of secreted frizzled-related protein 4 transgenic mice and analysis of their biological properties

Hua GUAN; Huiyuan ZHEN; Enqi LIU; Lixian XU; Qi YU

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Establishment and identification of secreted frizzled-related protein 4 transgenic mice and analysis of their biological properties

VernacularTitle:分泌卷曲相关蛋白4肝脏高表达转基因小鼠的制作及鉴定分析
Author: Hua GUAN ^{1
,

2} ; Huiyuan ZHEN ³ ; Enqi LIU ⁴ ; Lixian XU ⁵ ; Qi YU ¹
Author Information

1. Key Laboratory of Ischemic Cardiovascular Disease in Shaanxi & Institute of Basic and Translational Medicine of Xi’an Medical College, Xi’an 710021
2. Department of Anesthesiology, The Fourth Military Medical University Stomatological Hospital, Xi’an 710032
3. Department of Histology and Embryology, Xi’an Medical College, Xi’an 710021
4. Laboratory for Lipid Metabolism and Atherosclerosis, Xi’an Jiaotong University Cardiovascular Research Center, Xi’an 710061, China
5. Department of Anesthesiology, The Fourth Military Medical University Stomatological Hospital, Xi’an 710032
Publication Type:Journal Article
Keywords: secreted frizzled-related protein 4; transgenic mouse; plasma; liver
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2021;42(2):328-332
CountryChina
Language:Chinese
Abstract: 【Objective】 To generate secreted frizzled-related protein 4 (SFRP4) transgenic mice and analyze their biological properties. 【Methods】 We generated SFRP4 transgenic mice by DNA microinjection and detected the expression of SFRP4 by qRT-PCR and Western blotting. 【Results】 SFRP4 transgenic mice were successfully generated by DNA microinjection. The expression of SFRP4 was dramatically increased in transgenic mice liver compared with that of wide type. 【Conclusion】 The transgenic mice model of SFRP4 was successfully established by DNA microinjection. It provides a good model for studying the function of SFRP4 and the mechanism of participating in metabolic diseases.