LncRNA-TDRG1 facilitates the malignant biological behavior of cervical cancer cells

Yang Fan; Minghui Liu; Fengxiang Zhang; Minge Zhang; Kening Tian; Huafeng HE; Fang Wang; Yuliang Zou

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LncRNA-TDRG1 facilitates the malignant biological behavior of cervical cancer cells

VernacularTitle:LncRNA-TDRG1促进宫颈癌细胞恶性生物学行为的实验研究
Author: Yang FAN ¹ ; Minghui LIU ¹ ; Fengxiang ZHANG ¹ ; Minge ZHANG ¹ ; Kening TIAN ¹ ; Huafeng HE ¹ ; Fang WANG ¹ ; Yuliang ZOU ²
Author Information

1. Department of Gynecology, Xi’an Aerospace General Hospital, Xi’an 710010
2. Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, China
Publication Type:Journal Article
Keywords: cervical cancer; long non-coding RNA; TDRG1; Malignant biological behavior
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2021;42(2):245-250
CountryChina
Language:Chinese
Abstract: 【Objective】 To investigate the molecular mechanism of long non-coding RNA (lncRNA) TDRG1 in facilitating the malignant progression and poor prognosis of patients with cervical cancer. 【Methods】 Cervical cancer cell lines and normal cervical cell Ect1/E6E7 were collected. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of TDRG1. Cervical cancer cell lines were transfected with TDRG1-siRNA, and the proliferation of cancer cells was detected by CCK-8 method and cell plate cloning experiment. The invasion and migration of cancer cells were measured by Transwell experiment. The apoptosis of cancer cells was examined by flow cytometry, and the expressions of relevant proteins were tested by Western blot. 【Results】 Compared with Ect1/E6E7, cervical cancer cell lines showed relatively increased expression of TDRG1. Downregulation of TDRG1 expression inhibited the proliferation and colony formation (162±21 vs. 411±33, P<0.05), as well as the invasion and migration (invasion: 86±13 vs. 315±38, P<0.01; migration: 177±22 vs. 406±41, P<0.01) of Hela cells. Meanwhile, the apoptosis of Hela cells increased [(28±1.5)% vs. (16±1.2)%, P<0.05] and the expression of Bcl-2 protein reduced. In addition, TDRG1 knockdown also decreased the activity of autophagy in Hela cells. 【Conclusion】 TDRG1 facilitates the malignant biological progression of cervical cancer by inhibiting the apoptosis and providing a protective autophagy in cervical cells.