RYBP activates PARP-1 induced Parthanatos in esophageal squamous cell carcinoma cells and enhances response to YM155

Yue KE; Yuxing LI; Xiaobo Shi; Wei Guo; Xiaoxiao Liu; Yuchen Wang; Qinli Ruan; Jiyuan Pan; Xiaoping Yang

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RYBP activates PARP-1 induced Parthanatos in esophageal squamous cell carcinoma cells and enhances response to YM155

VernacularTitle:RYBP通过激活PARP-1 诱导Parthanatos增加食管鳞癌细胞对YM155的敏感性
Author: Yue KE ¹ ; Yuxing LI ¹ ; Xiaobo SHI ¹ ; Wei GUO ¹ ; Xiaoxiao LIU ¹ ; Yuchen WANG ¹ ; Qinli RUAN ¹ ; Jiyuan PAN ¹ ; Xiaoping YANG ²
Author Information

1. Department of Radiotherapy, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710004, China
2. Department of Thoracic Surgery, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710004, China
Publication Type:Journal Article
Keywords: esophageal squamous cell carcinoma; RYBP; PARP-1; YM155; Parthanatos
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2021;42(2):233-237,256
CountryChina
Language:Chinese
Abstract: 【Objective】 To explore the role of RYBP in activating PARP-1 dependent Parthanatos and promoting response to YM155 in esophageal squamous cell carcinoma. 【Methods】 CCK-8 and flow cytometry were used to analyze the inhibition ratio and cell death percentage after YM155 treatment in both RYBP overexpression group and control group. Western blotting was used to detect the expression of Parthanatos-related proteins. 【Results】 Compared with control group, RYBP overexpression group showed higher inhibition ratio and cell death percentage after YM155 treatment. Overexpression of RYBP activated PARP-1 with or without YM155 treatment. Besides, after YM155 treatment, KYSE170-RYBP showed more PAR accumulation in the nucleus, AIF translocation from mitochondria to the nucleus than control cells. 【Conclusion】 RYBP can activate PARP-1/PAR/AIF-dependent induced Parthanatos in esophageal squamous cell carcinoma and enhance response to YM155.