Network pharmacology predicts the mechanism and related experimental research on the effective components of Salvia miltiorrhiza and Carthamus tinctorius against cerebral ischemic stroke

Huiyuan Zhu; Qi Miao; Jiang Wang; Bin Luo; Haitong Wan; Wenxuan Wang; Bingyao Dong; Shengbin Xiao; Shan Dang

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Network pharmacology predicts the mechanism and related experimental research on the effective components of Salvia miltiorrhiza and Carthamus tinctorius against cerebral ischemic stroke

VernacularTitle:网络药理学预测丹红有效组分配伍抗缺血性脑卒中的作用机制及相关实验研究
Author: Huiyuan ZHU ¹ ; Qi MIAO ¹ ; Jiang WANG ¹ ; Bin LUO ¹ ; Haitong WAN ² ; Wenxuan WANG ¹ ; Bingyao DONG ³ ; Shengbin XIAO ⁴ ; Shan DANG ⁵
Author Information

1. School of Basic Medicine, Shaanxi University of Chinese Medicine, Xianyang 712046
2. School of Biological Engineering, Zhejiang Chinese Medical University, Hangzhou 310053
3. Department of Pain, The Second Affiliated Hospital of Xi’an Medical College, Xi’an 710068
4. Department of Statistics, Xi’an Jiaotong University Health Science Center, Xi’an 710061
5. Department of Geratology, Shaanxi Provincial People’s Hospital, Xi’an 710068, China
Publication Type:Journal Article
Keywords: network pharmacology; Salvia miltiorrhiza; Carthamus tinctorius; cerebral ischemic stroke; mechanism of action
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2021;42(3):474-483
CountryChina
Language:Chinese
Abstract: 【Objective】 To explore the effect mechanism of Salvia miltiorrhiza and safflower on combined anti-ischemic stroke and verify relevant action targets in middle cerebral artery occlusion (MCAO) rat model based on network pharmacology. 【Methods】 ①Traditional Chinese Medicine Systems Pharmacology (TCMSP) and GeneCards databases were used to screen the active components, component targets and ischemic stroke targets of Salvia miltiorrhiza and safflower respectively. The above data were imported into STRING database for protein interaction network analysis, and Cytoscape3.8.0 software was used to construct protein interaction network (PPI) and component target interaction network. Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway annotation analysis of target genes were performed using David online analysis tool. ② In this experiment, a rat model of ischemic stroke was prepared by using improved MCAO method, and immunohistochemical method and Real-time quantitative polymerase chain reaction (REAL-TIME PCR) to detect the positive expressions of NLRP3 inflammatory body and NF P65 protein in the brain tissue of rats in each group so as to explore the functional mechanism of anti-inflammation reaction against cerebral ischemia injury. 【Results】 ① A total of 87 effective components, corresponding to 253 targets, 1448 targets for ischemic stroke and 161 targets related to drugs and diseases, were screened from the Salvia milticorrhiza and safflower drug pairs. We obtained 730 biological processes, 81 cell components and 128 molecular functions through GO analysis, and 127 signal pathways through KEGG analysis. ②Immunohistochemical method and Real-time PCR determination results showed that compared with control group rats, model group rats had significantly increased tissue NLRP3 inflammatory body and NFkBp65 protein expressions (P<0.01). Compared with those in the model group, NLRP3 inflammatory body and NFkBp65 protein expressions significantly decreased in Dan red compatibility groups and nim horizon groups (P<0.01). 【Conclusion】 Compatibility of effective components in salvia miltiorrhiza, and carthamus tinctorius can further downregulate the release of inflammatory corpuscle NLRP3 through NFkB signaling pathway by blocking inflammatory lesions and thus plays the role of fighting against inflammatory damage.