Effect of Arntl on T cell development and anti-infection function in mice

Ya’e Sun; Anjun Jiao; Xin Wang; Xingzhe Zhang; Lei Lei; Xiaofeng Yang; Tao Xie; Xiaobo Zhou; Lin Shi; Baojun Zhang; Xiaobin Liu

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Effect of Arntl on T cell development and anti-infection function in mice

VernacularTitle:Arntl基因敲除对小鼠T细胞发育及抗感染功能的影响
Author: Ya’e SUN ^{1
,

2} ; Anjun JIAO ^{3
,

4} ; Xin WANG ^{3
,

4} ; Xingzhe ZHANG ^{3
,

4} ; Lei LEI ^{3
,

4} ; Xiaofeng YANG ^{3
,

4} ; Tao XIE ^{3
,

4} ; Xiaobo ZHOU ^{3
,

4} ; Lin SHI ^{3
,

4} ; Baojun ZHANG ^{3
,

4
,

5
,

6} ; Xiaobin LIU ¹
Author Information

1. Medical College of Yan’an University, Yan’an 716000
2. Department of Pathogenic Microbiology and Immunology, School of Basic Medical Sciences, Xi’an Jiaotong University, Xi’an 710061
3. Department of Pathogenic Microbiology and Immunology, School of Basic Medical Sciences, Xi’an Jiaotong University, Xi’an 710061
4. Institute of Infection and Immunity, Translational Medicine Institute, Xi’an Jiaotong University Health Science Center, Xi’an 710061
5. Key Laboratory of Environment and Genes Related to Diseases (Xi’an Jiaotong University), Ministry of Education, Xi’an 710061
6. Xi’an Key Laboratory of Immune Related Diseases, Xi’an 710061, China
Publication Type:Journal Article
Keywords: Arntl; T cell development; viral infection; immune response; Th cell differentiation
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2021;42(3):402-407,432
CountryChina
Language:Chinese
Abstract: 【Objective】 To evaluate the effect of Arntl on T cell development and T cell-mediated anti-viral immunity. 【Methods】 Arntl^F/FCD4cre⁺(KO) in mice was constructed to delete Arntl gene specifically in T cells. We examined the percentage and number of T cell subsets in the thymus and spleen by flow cytometry (FCM). At day 8 after lymphocytic choriomeningitis virus (LCMV) infection, the proportions of T cell subsets, virus-specific CD8⁺ T cells and IFN-γ secreting T cells were analyzed. The viral load in the spleen was measured using qPCR. Naive CD4⁺ T cells (CD4⁺CD25^-CD44^-CD62L⁺) were sorted by flow cytometry to perform T helper cell differentiation in vitro. 【Results】 The percentage and number of T cells in the thymus and spleen of KO mice showed no significant change compared with those in the control group (Arntl^F/FCD4cre^- mice, WT) (P>0.05). Acute LCMV infection did not cause observable changes in effector T cell proportion in the spleen of KO mice compared to that in WT mice (P>0.05), but KO mice showed a higher proportion of IFN-γ secreting T cells (P<0.05) and better virus clearance (P<0.05). In addition, naive CD4⁺ T cells from KO mice were more prone to differentiate into Th1 cells in vitro (P<0.05). 【Conclusion】 Arntl deletion in T cells does not affect T cell development, but enhances their ability to defend against viral infection by promoting Th1 cell differentiation and response.