Arctigenin regulates the effect of miR-21 on hypoxia-reoxygenation-induced pyroptosis of cardiomyocytes

Xiaoxia Yao; Zhirui Chen; Jing Lei; Ping Tang

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Arctigenin regulates the effect of miR-21 on hypoxia-reoxygenation-induced pyroptosis of cardiomyocytes

VernacularTitle:牛蒡子苷元调控miR-21对低氧复氧诱导的大鼠心肌细胞焦亡的影响
Author: Xiaoxia YAO ¹ ; Zhirui CHEN ² ; Jing LEI ³ ; Ping TANG ⁴
Author Information

1. School of Basic Medicine, Xinjiang Medical University, Urumqi 830000
2. Chongqing Yaoyou Pharmaceutical Co., Ltd., Chongqing 400000
3. Equipment Department of Bijie First People’s Hospital, Bijie 551700
4. School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200000, China
Publication Type:Journal Article
Keywords: arctigenin; miR-21; ischemia-reperfusion injury; cardiomyocyte; pyroptosis
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2022;43(5):763-768
CountryChina
Language:Chinese
Abstract: 【Objective】 To investigate the effect and mechanism of arctigenin (ARG) on hypoxia-reoxygenation (H/R) induced pyroptosis of cardiomyocytes. 【Methods】 H9C2 cells were cultured in vitro, and underwent hypoxia for 2 hours and reoxygenation for 4 hours to establish H/R cell injury model. The cells were divided into control group (Control), model group (H/R), ARG group, miR-21 simulation group (miR-21 mimic), and ARG+miR-21 inhibitor group (ARG+miR-21 inhibitor). TUNEL staining was used to detect the pyroptosis index of H9C2 cells; the lactate dehydrogenase (LDH) kit was used to detect the release of LDH in each group of cells; the enzyme-linked immunosorbent assay (ELISA) was used to detect the content of interleukin-1β (IL-1β) and interleukin-18 (IL-18). Western blotting was used to detect the expressions of pyroptosis-related proteins (Caspase-1, GSDMD, IL-1β and IL-18) in each group. 【Results】 Compared with those in the control group, the pyroptosis index, the release of LDH, IL-1β and IL-18, and the protein expressions of Caspase-1p20, GSDMD-N, IL-1β and IL-18 in the H/R group were significantly increased (P<0.01). Compared with H/R group, ARG group and miR-21 mimic group had significantly reduced pyroptosis index, LDH, IL-1β and IL-18 release, and protein expressions of Caspase-1 p20, GSDMD-N, IL-1β and IL-18 (P<0.01), and the above-mentioned index changes could be reversed after treatment with +miR-21 inhibitor. 【Conclusion】 ARG can inhibit H/R-induced pyroptosis of cardiomyocytes, and its mechanism is related to the promotion of miR-21 expression.