Comparison of macrolide resistance, molecular characteristics and MAST types of Bordetella pertussis collected from Xi’an and Shanghai

Juansheng Zhang; Diqiang Zhang; Chen Lin; Ling Chang; Chaofeng MA; Baozhong Chen; Mao Geng

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Comparison of macrolide resistance, molecular characteristics and MAST types of Bordetella pertussis collected from Xi’an and Shanghai

VernacularTitle:西安市与上海市百日咳鲍特菌对大环内酯类抗生素的耐药性、耐药相关分子特征及多位点抗原序列分型的比较研究
Author: Juansheng ZHANG ¹ ; Diqiang ZHANG ² ; Chen LIN ³ ; Ling CHANG ¹ ; Chaofeng MA ¹ ; Baozhong CHEN ¹ ; Mao GENG ⁴
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1. Xi’an Center for Disease Control and Prevention, Xi’an 710054
2. Pingchuan District People’s Hospital, Baiyin 730900
3. Shanghai Pudong New Area Center for Disease Control and Prevention, Shanghai 200120
4. Xi’an Yanta District Electronic City Community Health Service Center, Xi’an 710054, China
Publication Type:Journal Article
Keywords: Borde tella pertussis; macrolide resistance; genotype; molecular characteristic; multilocus antigen sequence typing (MAST)
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2022;43(5):691-696
CountryChina
Language:Chinese
Abstract: 【Objective】 To compare the macrolide resistance, molecular characteristics and multilocus antigen sequence typing (MAST) of Bordetella pertussis (Bp) collected from Xi’an and Shanghai so as to provide reference for prevention of pertussis and optimize vaccination strategies. 【Methods】 Erythromycin, azithromycin and clarithromycin susceptibility of clinical isolates collected from Xi’an and Shanghai during 2018 and 2019 were determined by E-test. PCR was used to detect the drug-resistant genes and mutation sites. MAST was employed to do molecular typing for the strains. The differences in macrolide resistance and MAST types between Xi’an and Shanghai were compared. 【Results】 Totally 34 strains from Xi’an and 26 strains from Shanghai were isolated. There were differences between Xi’an and Shanghai in the macrolide resistance (χ²=13.650, P<0.001). The composition ratio of MAST types of pertussis strains was also different between Xi’an and Shanghai (χ²=18.642, P<0.001) in that the prn1/ptxP1/ptxA1/fim3-1/fim2-1 strains dominated in Xi’an, while the prn1/ptxP1/ptxA1/fim3-1/fim2-1 and prn2/ptxP3/ptxA1/fim3-1/fim2-1 were almost half and half in Shanghai. A2047G site mutation was detected in all the macrolide-resistant strains, but not in all sensitive strains. Methylase genes ermA and ermB were detected in some macrolide-resistant strains. No other macrolide-resistant genes were found in resistant strains and no mutation or drug resistance gene was found in all the susceptible strains. 【Conclusion】 Differences existed between Xi’an and Shanghai in the macrolide resistance and MAST types of Bordetella pertussis strains. Further monitoring of Bordetella pertussis in China is required to better understand the resistance and evolution of the pathogen.