Analysis of chemical constituents and components absorbed into plasma of Ardisia crenata based on UPLC-QE-HF-MS/MS
- VernacularTitle:基于UPLC-QE-HF-MS/MS技术分析红凉伞化学成分及入血成分
- Author:
Hui SHI
1
,
2
,
3
;
Xiao LI
1
;
Ying ZHOU
1
,
2
;
Jingxin DING
1
;
Chang LIU
1
,
2
;
Xiongwei LIU
1
,
2
;
Xiu DONG
4
;
Yun CHEN
1
;
Tingting FENG
1
Author Information
1. School of Pharmacy，Guizhou University of Traditional Chinese Medicine，Guiyang 550025，China
2. Research Center for Application and Development of Medicine and Food Dual-use Resources，Guizhou University of Traditional Chinese Medicine，Guiyang 550025，China
3. Nano-drug Technology Research Center of Guizhou University of Traditional Chinese Medicine，Guiyang 550025，China
4. Guizhou Sanli Pharmaceutical Co.，Ltd.，Guizhou Anshun 561100，China
- Publication Type:Journal Article
- Keywords:
Ardisia crenata;
chemical constituents
- From:
China Pharmacy
2024;35(3):316-321
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To analyze the chemical constituents and components absorbed into plasma of the extract of Ardisia crenata and to elucidate its possible pharmacodynamic material basis. METHODS Overall， 12 rats were randomly assigned to the blank group （n＝6） and A. crenata group （n＝6） by the paired comparison method. The drug was administered once daily in the morning and afternoon for three days. Serum samples were prepared from serum after redosing on 4th day. The UPLC-QE-HF-MS/ MS was used to analyze and identify the chemical constituents in A. crenata extract and serum samples. Compound Discoverer 3.0 was employed for retention time correction， peak identification， and peak extraction. According to the secondary mass spectrometry information， the Thermo mzCloud online and Thermo mzVault local databases， referring to the relevant literature and control quality spectrum information were used to preliminarily identify the chemical constituents and components absorbed into plasma of A. crenata. RESULTS A total of 34 compounds were identified from the extract of A. crenata， mainly coumarins， flavonoids， organic acids， amino acids， including bergenin， quercetin， gallic acid， L-pyroglutamic acid， etc. Besides， 5 components absorbed into plasma were identified from serum samples: L-pyroglutamic acid， syringic acid， bergenin， cinnabar root saponin A， and mycophenolic acid. CONCLUSIONS L-pyroglutamic acid， syringic acid， bergenin， cinnabar root saponin A， and mycophenolic acid may act as the pharmacodynamic material basis of A. crenata.
