Yunkang Oral Liquid Promotes Restoration of Mice from Postpartum Kidney Deficiency via TGF-β/Smads Signaling Pathway
10.13422/j.cnki.syfjx.20240112
- VernacularTitle:孕康口服液通过TGF-β/Smads信号通路调节产后肾虚小鼠的复旧作用分析
- Author:
Jiayi ZHENG
1
;
Jie SU
1
;
Meiqiu YAN
1
;
Jingjing YU
1
;
Yigong CHEN
1
;
Meng FU
1
;
Xueling HU
1
;
Mengxi CHANG
1
;
Guiyuan LYU
1
;
Liqin JIANG
1
Author Information
1. Zhejiang Chinese Medical University， Hangzhou 311402， China
- Publication Type:Journal Article
- Keywords:
Yunkang oral liquid;
postpartum mice;
kidney deficiency;
mechanism
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(6):83-90
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of Yunkang oral liquid on postpartum kidney deficiency in mice. MethodPostpartum mice were randomized into model and low-dose （6 mL·kg-1）， medium-dose （9 mL·kg-1）， and high-dose （12 mL·kg-1） Yunkang oral liquid groups. The mouse model of postpartum kidney deficiency was established by sleep deprivation combined with forced swimming. Another 9 female ICR mice were selected as the normal control group. The mice were administrated with Yunkang oral liquid during the period of modeling. The levels of estradiol （E2）， progesterone （P）， follicle-stimulating hormone （FSH）， and luteinizing hormone （LH） in the serum were measured by the enzyme-linked immunosorbent assay. The morphological changes of ovaries and uterus were observed by hematoxylin-eosin （HE） staining， and the expression of transforming growth factor （TGF）-β and Smad2/3 was determined by immunohistochemistry and Western blotting. ResultThe mice in the model group showed prolonged estrous cycle， reduced voluntary activity， dorsal temperature， grip strength， and bone strength， and whitening tongue coating. Compared with the model group， Yunkang oral liquid shortened the estrous cycle， increased the voluntary activity， dorsal temperature， grip strength， and bone strength， and alleviated the whitening of tongue coating. Moreover， it elevated the E2 and P levels and lowered the FSH and LH levels in the serum， decreased ovarian follicular atresia rate， promoted uterine repair， and down-regulated the expression of TGF-β and Smad2/3 in the ovarian and uterine tissues. ConclusionYunkang oral liquid can ameliorate postpartum kidney deficiency in mice by regulating the TGF-β/Smads signaling pathway.
