Phytochemical profile and nematicidal activity of a hydroalcoholic extract from Cazahuate flowers (Ipomoea pauciflora M. Martens & Galeotti) against Haemonchus contortus infective larvae
https://doi.org/10.47665/tb.40.1.017
- Author:
Delgado-Núñez, E.J.
1
;
López-Arellano, M.E.
2
;
Olmedo-Juárez, A.
2
;
Díaz-Nájera, J.F.
3
;
Ocampo-Gutiérrez, A.Y.
2
;
Mendoza-de Gives, P.
2
Author Information
1. Faculty of Agricultural, Livestock and Environmental Sciences, Autonomous University of Guerrero, Iguala, Guerrero, Mexico, CP 40040
2. National Center for Disciplinary Research in Animal Health and Safety. (CENID-SAI-INIFAP), Jiutepec, Morelos, Mexico, CP 62574
3. Department of Phytotechnics, Agricultural College from the State of Guerrero, Cocula, Guerrero, Mexico, CP 40000
- Publication Type:Journal Article
- Keywords:
Nematocidal;
Cazahuate;
Haemonchus;
phytocompounds.
- From:Tropical Biomedicine
2023;40(No.1):108-114
- CountryMalaysia
- Language:English
-
Abstract:
Haemonchus contortus (Hc) is a hematophagous parasite affecting the health and productivity of
flocks. The administration of chemical anthelmintic drugs (AH) is the common method of deworming;
however, generates resistance in the parasites to AH and it is a public health risk due to drug residues in
milk, meat and sub-products. Natural compounds from plants are explored to diminish this parasitosis,
improving their health and productivity, without the negative effects of AH. Ipomoea genus is a group
of climbing plants belonging to the Convulvulaceae family possessing perennial leaves and tuberous
roots. Medicinal properties has been attributed to this plant including nutritional agents, emetics,
diuretics, diaphoretics, purgatives and pesticides. The objective of this study was assessing the in vitro
nematocidal activity of a hydroalcoholic extract (HA-E) obtained from Ipomoea pauciflora (Cazahuate)
flowers against Hc infective larvae (L3) and to identify its phytochemical profile (PhC-P). The assay was
carried out using microtiter plates (MTP). Four HA-E concentrations were assessed and Ivermectin and
distilled water were used as positive and negative control groups, respectively. Approximately 100 Hc
L3 were deposited in each well (n=12) and incubated at 25–35°C for 7 days. Data were analyzed using
ANOVA and a General Linear Model (GLM) followed by Tukey test (P<0.05). The treatments showing a
concentration-dependent effect (CDE) were analyzed to identify their 50% and 90% lethal concentrations
(CL50, 90) via a Probit Analysis. The highest mortality was observed at 50 mg/mL (82.64 ± 0.71%) and
the lowest at 6.25 mg/mL (56.46 ± 2.49%), showing a CDE with increasing mortality from 6.25 to 50
mg/mL. The PhC-P revealed the presence of alkaloids, coumarins, flavonoids, tannins and triterpenes/
sterols. A HA-E from flowers of I. pauciflora will be considered to assess its potential use in the control
of haemonchosis in small ruminants.
- Full text:8.2023my1395.pdf
