Establishment of a rat model with aortic dissection induced by β-aminopropionitrile combined with angiotensin Ⅱ
- VernacularTitle:β-氨基丙腈联合血管紧张素Ⅱ诱导大鼠主动脉夹层模型的建立
- Author:
Yi ZHANG
1
;
Xinmei LIU
2
;
Guangqi ZHOU
3
;
Yanli QIAO
4
;
Haiyang HU
1
;
Hongsheng LIU
2
Author Information
1. Clinical Medical College of Jining Medical University, Jining, 272013, Shandong, P. R. China
2. Department of Cardiac Intensive Medicine, Affiliated Hospital of Jining Medical University, Jining, 272029, Shandong, P. R. China
3. Department of Breast Surgery, Affiliated Hospital of Jining Medical University, Jining, 272029, Shandong, P. R. China
4. Department of Cardiac Surgery, Affiliated Hospital of Jining Medical University, Jining, 272029, Shandong, P. R. China
- Publication Type:Journal Article
- Keywords:
β-aminopropionitrile;
angiotensin Ⅱ;
aortic dissection;
rat;
model;
complications
- From:
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
2024;31(01):127-134
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the optimal administration combination of β-aminopropionitrile (BAPN) and Angiotensin Ⅱ (Ang-Ⅱ) in the establishment of SD rat aortic dissection (AD) model and the related complications. Methods Forty-two three-week-old male SD rats were randomly divided into 7 groups: a group A (0.25% BAPN), a group B (0.40% BAPN), a group C (0.80% BAPN), a group D [1 g/(kg·d) BAPN], a group E [1 g/(kg·d) BAPN+ 1 μg/(kg·min) saline], a group F [1 g/(kg·d) BAPN+1 μg/(kg·min) Ang-Ⅱ] and a group G (control group). There were 6 rats in each group. The intervention period was 4 weeks (groups E and F were 4 weeks+5 days). Rats were dissected immediately if they died during the experiment. After the intervention, the surviving rats were sacrificed by pentobarbital sodium, and the whole aorta was separated and retained. Hematoxylin-eosin staining was used to observe the changes of aorta from the pathological morphology. Results There was no statistical difference in the survival rate among the groups after 4 weeks of BAPN intervention (P>0.05). After 5 days of mini-osmotic pumps implantation, the survival rate of rats was higher in the group E than that in the group F (P=0.008), and the incidence of AD in the group E was lower than that in the group F (P=0.001). BAPN could affect the food and water intake of rats. After BAPN intervention for 4 weeks, the body weight of rats in the group G was higher than those in the intervention groups (P<0.05). BAPN combined with Ang-Ⅱ could make the aortic intima thick, elastic fiber breakage, arrangement disorder, and inflammatory cell infiltration in rats, which conformed to the pathological and morphological changes of AD. BAPN could also affect mental state and gastrointestinal tract. Conclusion The combination of BAPN [1 g/(kg·d)] and Ang-Ⅱ [1 μg/(kg·min)] can stably establish AD model in rats, which will provide a stable carrier for further study of the pathogenesis and therapeutic targets of AD. However, the complications in this process are an unstable factor. How to balance the influence of BAPN on other tissues and organs in the process of AD model establishment remains to be further studied.
