Plasmodium knowlesi circumsporozoite protein: genetic characterisation and predicted antigenicity of the central repeat region
https://doi.org/10.47665/tb.40.1.004
- Author:
Tan, J.H.
1
;
Cheong, F.W.
1
;
Lau, Y.L.
1
;
Fong, M.Y.
1
Author Information
1. Department of Parasitology, Faculty of Medicine, Universiti Malaya, 50603 Kuala Lumpur, Malaysia
- Publication Type:Journal Article
- Keywords:
Circumsporozoite protein;
central repeat;
P. knowlesi;
genetic characterisation;
B-cell epitopes.
- From:Tropical Biomedicine
2023;40(No.1):37-44
- CountryMalaysia
- Language:English
-
Abstract:
Circumsporozoite protein (CSP) central repeat region is one of the main target regions of the RTS,S/AS01
vaccine for falciparum infection as it consists of immunodominant B cell epitopes. However, there is a lack
of study for P. knowlesi CSP central repeat region. This study aims to characterise the CSP repeat motifs
of P. knowlesi isolates in Peninsular Malaysia. CSP repeat motifs of 64 P. knowlesi isolates were identified
using Rapid Automatic Detection and Alignment of Repeats (RADAR). Antigenicity of the repeat motifs
and linear B cell epitopes were predicted using VaxiJen 2.0, BepiPred-2.0 and BCPred, respectively. A total
of 35 dominant repeat motifs were identified. The repeat motif “AGQPQAQGDGANAGQPQAQGDGAN”
has the highest repeat frequency (n=15) and antigenicity index of 1.7986. All the repeat regions were
predicted as B cell epitopes. In silico approaches revealed that all repeat motifs were antigenic and
consisted of B cell epitopes which could be designed as knowlesi malaria vaccine.
- Full text:8.2023my1387.pdf
