Distribution characteristics of antibiotic resistance genes in the domestic water of residents in Haimen， Jiangsu Province
10.19428/j.cnki.sjpm.2023.23158
- VernacularTitle:江苏省海门市某村生活用水相关水环境中抗生素抗性基因的分布
- Author:
Chuanwu MAO
1
;
Li ZHANG
1
;
Jinxin ZANG
1
;
Lufang JIANG
1
;
Chenglong XIONG
1
;
Na WANG
1
;
Feng JIANG
1
;
Chaowei FU
1
;
Jingjing HU
2
;
Qingwu JIANG
1
Author Information
1. School of Public Health， Fudan University， Shanghai 200032， China
2. Shanghai Pinnacles Medical Technology Co.， Shanghai 201114， China
- Publication Type:Journal Article
- Keywords:
antibiotic resistance gene;
metagenomics;
microbial diversity;
water environment
- From:
Shanghai Journal of Preventive Medicine
2023;35(12):1199-1205
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveAntibiotic resistance genes （ARGs） have received wide attention all over the world. The purpose of this study was to explore the bacterial community structure， the types and levels of antibiotic resistance genes in a water body in east China， and to compare and analyze the characteristics of microbial species distribution and antibiotic resistance gene distribution in various water environments. MethodsA total of 10 households in Haimen City， Jiangsu Province were selected and their surrounding water environment samples were collected. 21 water samples including river water （4）， Mingou water （9） and well water （8） were collected for metagenomics sequencing， assembled with MetaWRAP， annotated with CARD database， and analyzed with R software. ResultsIn various water bodies， the dominant bacteria phyla was Proteobacteria， the dominant bacteria genera were Deuterostomia， Pseudomonas， Flavobacteriales and Streptomycetaceae. The ARGs annotated were mainly composed of quinolones， aminoglycosides， macrolides and beta-lactams antibiotic resistance genes. The top four relative abundance of resistance genes were macB， RanA， evgS and TxR， The average absolute abundance and expression of resistance genes in well water and Mingou water were higher than those in river water. ConclusionMultiple ARGs are detected to varying degrees in well water， river water， and Mingou water bodies， and the expression of resistance genes in well water and Mingou water bodies is higher than that in river water bodies， possibly due to human production and living activities.
