Correlation between intestinal flora characteristics and hepatic and renal impairments in HIV-infected heterosexual men
10.19428/j.cnki.sjpm.2023.23322
- VernacularTitle:男性感染HIV异性性行为者的肠道菌群特征与肝肾功能损伤的相关性
- Author:
Mingjun MA
1
;
Jialu HUANG
1
;
Lidan ZHU
2
;
Panpan CHEN
2
;
Yong ZHANG
2
;
Haoran ZHANG
1
;
Qiuli XU
2
;
Qianqian LIU
2
;
Shaotan XIAO
1
;
Xin XIN
1
Author Information
1. School of Public Health， Dali University， Dali， Yunnan 671000， China
2. Pudong New Area Center for Disease Control and Prevention， Pudong Institute of Preventive Medicine， Fudan University， Shanghai 200136， China
- Publication Type:Journal Article
- Keywords:
HIV infection;
16S rDNA;
liver function;
kidney function;
intestinal flora;
heterosexual men
- From:
Shanghai Journal of Preventive Medicine
2023;35(12):1192-1198
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the relationship between the intestinal flora and the impairment of liver and kidney in HIV-infected men who have heterosexual sex with healthy women. MethodsFecal samples from 41 HIV-infected heterosexual men who have sex with women （PMSW） and 43 age- and BMI-matched healthy heterosexual men who have sex with women （NMSW） were collected and subjected to 16S rDNA sequencing. The blood levels of AST， ALT， TBIL， UREA， Cr， UA， β2-MG and other liver and kidney function indicators were measured. Bioinformatics methods were used to analyze the characteristics of the intestinal flora of the patients in these two groups， to compare the differential bacteria strains， and to analyze their correlation with liver and kidney function indicators. ResultsIn comparison with NMSW， the alpha diversity of intestinal flora was decreased in PMSW， and the beta diversity analysis showed significant differences in flora characteristics between the two groups （P<0.05）. The abundance of Clostridium， Phylum thick-walled， Trichosporon， and Clostridium tumefaciens decreased but Fusobacteriota increased （LDA score >4）. The comparison of liver and kidney function indexes revealed that AST， β2-MG levels were higher in PMSW than in NMSW， while TBIL was lower in PMSW than in NMSW. The number of patients with abnormal β2-MG was much higher in PMSW than in NMSW， and the difference was statistically significant （P<0.001）. It was also found that AST was negatively correlated with Clostridium （P<0.05）； TBIL was negatively correlated with Clostridium and positively correlated with Phylum thick-walled and Trichosporon （P<0.05）. β2-MG was negatively correlated with Phylum thick-walled， Clostridium， Trichosporon and Rumenococcus （P<0.05） and positively correlated with Clostridium （P<0.05）. ConclusionIn PMSW group， the alpha diversity of the flora is decreased. AST and β2-MG levels are increased， and TBIL level is decreased. These changes were significantly correlated with different strains of bacteria in the intestinal flora.
