Establishment of a RILD model in C57BL/6J mice treated with precise radiotherapy
10.13491/j.issn.1004-714X.2023.06.003
- VernacularTitle:精准放疗下C57BL/6J鼠放射性肝损伤模型的建立
- Author:
Huihui XIAO
1
;
Lingling LIU
2
;
Yongkang ZHANG
2
;
Miaomiao YANG
3
,
4
;
Xiangli CUI
2
;
Zongtao HU
2
Author Information
1. Affiliated Cancer Hospital of Harbin Medical University, Harbin 150000 China.
2. Hefei Cancer Hospital, Chinese Academy of Sciences, Hefei 230000 China.
3. The First Affiliated Hospital of Anhui Medical University, Hefei 230000 China
4. Anhui Public Health Clinical Center, Hefei 230000 China.
- Publication Type:OriginalArticles
- Keywords:
Radiation-induced liver disease;
Stereotactic body radiation therapy;
C57BL/6J mouse;
Model
- From:
Chinese Journal of Radiological Health
2023;32(6):611-617
- CountryChina
- Language:Chinese
-
Abstract:
Objective To provide a reliable and stable animal model for investigating the molecular pathogenesis of radiation-induced liver disease (RILD). Methods Ninety C57BL/6J mice were divided into control, 20 Gy, 25 Gy, 30 Gy and 35 Gy radiation groups. The mice were executed at 4 weeks after radiation and the levels of alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase in the liver serum were measured. HE staining was performed on the pathological liver tissues. Masson staining was performed at 36 weeks after radiation. Results Compared with the control group, the fatality rate was higher in the 30 and 35 Gy radiation groups, and the body weight significantly decreased in the 20 and 25 Gy radiation groups. Compared with the control group, alanine aminotransferase significantly increased in mice exposed to 20 Gy, while aspartate aminotransferase and alanine aminotransferase increased in mice exposed to 25 Gy. No significant changes were observed in the livers of the mice in the 20 and 25 Gy radiation groups, but pathological examination showed liver damage induced by both 20 and 25 Gy radiation. Conclusion A stable and reliable mouse model of RILD was constructed for treatment with linear accelerator. The mouse model of RILD constructed for stereotactic body radiation therapy using linear accelerator has significant research implications for the exploration of RILD.
