Genetic Diversity Analysis and Molecular ID Card Construction of Pinellia ternata Based on Phenotypic Traits and SSR Markers
10.13422/j.cnki.syfjx.20231812
- VernacularTitle:基于表型性状和SSR标记的半夏遗传多样性分析及分子身份证构建
- Author:
Yike XU
1
;
Shuang LI
1
;
Peiwen KOU
1
;
Changle LIU
1
;
Xiaochun SUN
1
;
Wenjing HUANG
1
Author Information
1. Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization， State Key Laboratory of Research， Development of Characteristic Qin Medicinal Resources （Cultivation）， Shaanxi University of Chinese Medicine， Xianyang 712083， China
- Publication Type:Journal Article
- Keywords:
Pinellia ternata;
SSR molecular marker;
genetic diversity;
molecular ID card
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(5):134-142
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the genetic diversity and genetic relationship of Pinellia ternata germplasm resources and provide the basis for germplasm identification， variety breeding， and resource conservation. MethodIn this study， 27 P. ternata were used as experimental materials to determine seven phenotypic characters， such as plant height， leaf length， and leaf width. Simple sequence repeats （SSR） primers were designed based on P. ternata transcriptome data， and polymerase chain reaction （PCR） amplification was performed on 27 P. ternata samples. The genetic diversity of P. ternata germplasm was analyzed by POPGENE32， PowerMarker V3.25， and NTSYS-PC 2.10e software. ResultA total of 10 pairs of highly polymorphic primers （PIC>0.5） and four pairs of moderately polymorphic primers （0.25
