Preparation and in vivo Distribution of Essential Oil from Alpinia zerumbet Fructus Encapsulated Nanoemulsions
10.13422/j.cnki.syfjx.20231349
- VernacularTitle:艳山姜挥发油纳米乳的制备及其体内分布情况考察
- Author:
Jinzhuan XU
1
;
Lili ZHANG
1
;
Zhengli ZHOU
1
;
Shan XU
1
;
Xiang ZHOU
1
;
Lei HUANG
1
;
Zipeng GONG
1
;
Yi CHEN
1
;
Xiangchun SHEN
1
Author Information
1. Guizhou Provincial Engineering Center for Efficient Utilization of Natural Pharmaceutical Resources，Key Laboratory of Optimal Utilization of Natural Medicinal Resources，Guizhou Provincial Key Laboratory of Pharmaceutics，School of Pharmaceutical Sciences，Guizhou Medical University，Guiyang 550025，China
- Publication Type:Journal Article
- Keywords:
nanoemulsions;
proteoglycan;
vitamin B12（VB12）;
essential oil from Alpinia zerumbet fructus（EOFAZ）;
oral preparations;
in vivo distribution;
liver targeting
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(5):126-133
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo prepare oral nanoemulsions encapsulating essential oil from Alpinia zerumbet fructus（EOFAZ） and to investigate its pro-absorption effect in vitro and distribution in vivo. MethodThe proteoglycan conjugate polysaccharides of vinegar-processed Bupleuri Radix-bovine serum albumin（VBCP-BSA） was prepared by Maillard reaction of VBCP and BSA. Taking VBCP-BSA as emulsifier， vitamin B12（VB12） as absorption enhancer， and medium chain triglycerides mixed with EOFAZ as oil phase， the nanoemulsions loaded with EOFAZ was prepared by high energy emulsification method. The particle size， particle size distribution， surface Zeta potential， EOFAZ content and appearance and morphology of the nanoemulsions were characterized， and fluorescein tracer method was used to investigate the absorption effect of fluorescein-labeled EOFAZ nanoemulsions in vitro and their distribution in vivo. ResultVBCP-BSA was formed by Maillard reaction for 48 h with high grafting rate. Using VBCP-BSA as emulsifier， the homogeneous pink nanoemulsions was prepared and denoted as EOFAZ@VBCP-BSA/VB12. The particle size of the nanoemulsions was less than 100 nm and the particle size distribution was uniform. The surface of the nanoemulsions was a weak negative charge， and the shape was spherical. The encapsulation rate of the nanoemulsions for EOFAZ was greater than 80%， which had a good absorption effect in vitro and could enhance liver accumulation after oral administration. ConclusionThe designed proteoglycan nanoemulsions can effectively load EOFAZ， promote oral absorption and enhance liver distribution， which can provide experimental basis for the development of oral EOFAZ liver protection preparations.
