Oncolytic Newcastle disease virus inhibits IL-6 induced migration and invasion of human glioblastoma U87MG cells
DOI:10.3872/j.issn.1007-385x.2024.01.004
- VernacularTitle:溶瘤新城疫病毒抑制IL-6诱导的人胶质母细胞瘤U87MG细胞的迁移和侵袭
- Author:
TAO Weiyi
1
;
QIN Ying
1
;
WU Xing
1
;
ZHENG Tingting
1
;
FAN Xiaohui
1
;
LIANG Ying
1
Author Information
1. Guangxi Key Laboratory of Basic Research on Regional Diseases, Department of Microbiology, School of Basic Medicine, Guangxi Medical University, Nanning 530021, Guangxi, China
- Publication Type:Journal Article
- Keywords:
新城疫病毒;IL-6;胶质母细胞瘤;U87MG细胞;增殖;迁移;侵袭;JAK2/STAT3信号通路
- From:
Chinese Journal of Cancer Biotherapy
2024;31(1):27-31
- CountryChina
- Language:Chinese
-
Abstract:
[摘 要] 目的:探讨溶瘤新城疫病毒(NDV)对IL-6诱导的人胶质母细胞瘤U87MG细胞增殖、迁移和侵袭的作用及其可能的机制。方法:将U87MG细胞分为对照组、IL-6组、NDV组、NDV+IL-6组,其中IL-6组与NDV+IL-6组用75 ng/mL IL-6预处理1 h,其余组用DMEM预处理1 h,后分别用DMEM、75 ng/mL IL-6、1 HU NDV、1 HU NDV+75 ng/mL IL-6处理24 h。MTT法、细胞划痕实验和Transwell侵袭实验分别检测IL-6、NDV对U87MG细胞增殖、迁移和侵袭的影响,WB法检测各组细胞JAK2、p-JAK2、STAT3、p-STAT3和MMP2蛋白的表达水平。结果:与对照组相比,IL-6组细胞迁移率显著升高(P<0.05),侵袭细胞数目显著增多(P<0.01);与IL-6组相比,NDV+IL-6组U87MG细胞增殖率显著降低(P<0.05),细胞迁移率和侵袭细胞数目均显著降低(均P<0.01)。WB实验结果显示,与对照组相比,IL-6组p-STAT3/STAT3比值显著升高(P<0.01),NDV组p-JAK2/JAK2、p-STAT3/STAT3比值显著降低(P<0.05,P<0.01),MMP-2蛋白表达量显著降低(P<0.01);与IL-6组相比,NDV+IL-6组p-STAT3/STAT3比值、MMP-2蛋白表达量均显著降低(均P<0.05)。结论:NDV能抑制IL-6对人脑胶质瘤U87MG细胞迁移和侵袭的诱导作用,其机制可能与JAK2/STAT3信号通路的参与调控有关。
- Full text:20240104.pdf