Study on metabolites derived from Zhideke granules in rats in vivo
- VernacularTitle:止得咳颗粒在大鼠体内的代谢产物研究
- Author:
Jie LIANG
1
,
2
,
3
,
4
;
Piaoxue ZHENG
1
,
2
;
Huihua CHEN
1
,
2
;
Chunyan HUANG
5
;
Yanli LIANG
1
,
2
;
Chunlian LU
1
,
2
;
Jingjing XIE
1
,
2
;
Yuming MA
1
,
2
;
Jiawen PENG
1
,
2
;
Lichun ZHAO
4
;
Rilan CHEN
6
Author Information
1. School of Pharmacy，Guangxi University of Chinese Medicine，Nanning 530200，China
2. Guangxi Universities Laboratory of TCM Extraction，Purification and Quality Analysis，Nanning 530200，China
3. National Engineering Research Center South China Branch of TCM Solid Preparation Manufacturing Technology，Nanning 530200，China
4. Guangxi Zhuang Yao Medicine Engineering Technology Research Center，Nanning 530200，China
5. Qinzhou Inspection and Testing Center，Qinzhou 535000，China
6. Dept. of Pharmacy，Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine，Nanning 530011，China
- Publication Type:Journal Article
- Keywords:
Zhideke granules;
UPLC-Q-Exactive-MS
- From:
China Pharmacy
2024;35(2):172-178
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To analyze the metabolites of Zhideke granules and speculate its metabolic pathway in rats in vivo. METHODS Male SD rats were randomly divided into blank group and administration group （Zhideke granules， 9.45 g/kg）； they were given ultrapure water or relevant medicine， twice a day， every 6-8 h， for 3 consecutive days. Serum， urine and feces samples of rats were collected， and their metabolites were identified by UPLC-Q-Exactive-MS technique after intragastric administration of Zhideke granules； their metabolic pathways were speculated. RESULTS After intragastric administration of Zhideke granules， 16 prototype components （i.g. irisflorentin， baicalin， chlorogenic acid） and 11 metabolites （i.g. hydration products of kaempferol or luteolin， methylation products of chlorogenic acid， and hydroxylation products of baicalin） were identified in serum， urine and feces of rats. Among them， 8 prototype components and 4 metabolites were identified in serum samples； 10 prototype components and 7 metabolites were identified in urine samples； 8 prototype components and 5 metabolites were identified in the fecal samples. CONCLUSIONS The metabolites of Zhideke granules in rats mainly include baicalin， irisflorentin，chlorogenic acid， and the main metabolic pathways included methylation， hydroxylation， glucuronidation.
