Effects of immune infiltration-related gene CSAG1 on the progression of renal clear cell carcinoma

Wenheng BO; Tianzhen HE; Xueyu LI; Kai Zhao; Zongliang Zhang; Xinbao Yin; Zhenlin Wang; Yulian Zhang; Han Yang; Yuanming Sui; Qinglei Wang; Zhaofeng LI; Ke Wang

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Effects of immune infiltration-related gene CSAG1 on the progression of renal clear cell carcinoma

VernacularTitle:免疫浸润相关基因CSAG1对肾透明细胞癌进展作用的研究
Author: Wenheng BO ¹ ; Tianzhen HE ² ; Xueyu LI ¹ ; Kai ZHAO ¹ ; Zongliang ZHANG ¹ ; Xinbao YIN ¹ ; Zhenlin WANG ¹ ; Yulian ZHANG ¹ ; Han YANG ¹ ; Yuanming SUI ¹ ; Qinglei WANG ¹ ; Zhaofeng LI ¹ ; Ke WANG ¹
Author Information

1. Department of Urology, Affiliated Hospital of Qingdao University, Qingdao 266000
2. Institute of Special Medicine, Nantong University, Nantong 226019, China
Publication Type:Journal Article
Keywords: renal clear cell carcinoma; CSAG1; bioinformatics; immune infiltration; tumor microenvironment; prognosis; qRT-PCR; biomarker
From: Journal of Modern Urology 2023;28(3):247-253
CountryChina
Language:Chinese
Abstract: 【Objective】 To explore the correlation between CSAG1 expression and the prognosis and tumor-infiltrating lymphocytes in renal clear cell carcinoma (RCCC), and to predict the survival and tumor progression. 【Methods】 The gene expression profiles and clinical information of CSAG1 were downloaded from the Cancer Genome Atlas (TCGA). Based on the differential mRNA expression, GO annotation and KEGG pathway analysis were performed. The relationship between CSAG1 and tumor immune infiltration was assessed with Tumor Immunoassay Resource (Timer 2.0) database. The mRNA expression of CSAG1 in human RCCC specimens was validated with qRT-PCR. 【Results】 CSAG1 expression was significantly higher in RCCC tissues than in normal tissues (P<0.05). The qRT-PCR results revealed that the mRNA level of CSAG1 was consistent with that predicted by bioinformatic analysis. The KEGG analysis and GO annotation indicated high GSAG1 expression in RCCC was related to transmembrane transport, tricarboxylic acid cycle and lysosome. CSAG1 expression was positively related to the infiltration of pDC, aDC, CD8⁺ T cells, cytotoxic cells, TFH, TH1 cells, Tem, NK CD56^dm cells, Treg and T cells, but negatively correlated with macrophage infiltration. 【Conclusion】 CSAG1 may be associated with poor prognosis of RCCC and become a potential immunotherapy target.