Protective effects of diosmetin on renal ischemia-reperfusion injury in mice
10.3969/j.issn.1009-8291.2023.05.016
- VernacularTitle:香叶木素对小鼠肾缺血再灌注损伤的保护作用
- Author:
Jinjian ZHOU
1
;
Kang YANG
2
Author Information
1. Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan 430061, China
2. Department of Urology, Renmin Hospital of Wuhan University, Wuhan 430061, China
- Publication Type:Journal Article
- Keywords:
renal ischemia-reperfusion;
acute kidney injury;
diosmetin;
inflammatory response
- From:
Journal of Modern Urology
2023;28(5):441-444
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate whether diosmetin has protective effects on renal ischemia-reperfusion (I/R) injury in mice and explore the potential mechanism. 【Methods】 A total of 45 BALB/c male mice were randomly divided into the sham group, I/R group and diosmetin treatment group. The protective effects of diosmetin on the renal function of mice was evaluated by detecting blood biochemical indexes and renal tissue HE staining injury score. The expression of p-P65, a key molecule of NF-κB family, was detected with immunohistochemistry and western blotting, and the downstream inflammatory factors of NF-κB signaling pathway were detected with quantitative polymerace chain reaction (qPCR). 【Results】 Compared with the sham group, the I/R group had significantly increased levels of serum creatinine and urea nitrogen, significantly damaged morphological construction, and significantly increased expressions of p-P65 and inflammatory cytokines, while the diosmetin treatment group had decreased levels of serological indexes, improved structure of kidney tissue and reduced expressions of p-P65 and inflammatory cytokines. 【Conclusion】 Diosmetin can protect kidney from I/R-induced acute kidney injury by inhibiting NF-κB pathway in mice.
