Effects of chlorogenic acid on the proliferation, migration and invasion of renal carcinoma A498 and 769-P cells
10.3969/j.issn.1009-8291.2023.05.015
- VernacularTitle:绿原酸对肾癌A498和769-P细胞增殖、迁移和侵袭的影响
- Author:
Haibin CHEN
1
;
Lijiao WANG
2
;
Jianjun ZHAO
1
;
Chao TAN
1
Author Information
1. Department of Urology, Affiliated Hospital of Hebei Engineering University, Handan 056004, China
2. Department of Respiratory Medicine, Affiliated Hospital of Hebei Engineering University, Handan 056004, China
- Publication Type:Journal Article
- Keywords:
chlorogenic acid;
renal carcinoma A498 cells;
renal carcinoma 769-P cells;
migration and invasion;
EPAS-1
- From:
Journal of Modern Urology
2023;28(5):436-440
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the effects of chlorogenic acid on the proliferation, migration and invasion of renal carcinoma A498 and 769-P cells and the possible molecular mechanism. 【Methods】 Human renal carcinoma A498 and 769-P cells were divided into control group and chlorogenic acid group (2 μL,1 μmol/L) and cultured for 72 h. The cell proliferation, invasion and migration were detected with MTT assay, Transwell assay and scratch test, respectively. The expressions of IL-1β, EPAS-1 and AKT/P65 signaling pathway related proteins were detected with ELISA, qRT-PCR and Western blot, respectively. 【Results】 Chlorogenic acid inhibited the proliferation, invasion and migration of renal carcinoma A498 and 769-P cells, and reduced the IL-1β level in the cell supernatant. Anti-IL-1β reduced the protein and mRNA expressions of EPAS-1, p-AKT and p-P65. Compared with the control group, the chlorogenic acid group had reduced mRNA and protein expressions of EPAS-1, p-AKT and p-P65 (P<0.05). 【Conclusion】 Chlorogenic acid can inhibit the invasion and metastasis of renal carcinoma cells, and its mechanism may be related to inhibiting the secretion of IL-1β, thereby inhibiting the AKT/P65/EPAS-1 pathway.