Effects of histone acetyltransferase KAT7 inhibitor WM-3835 on the proliferation and migration of bladder cancer cells
10.3969/j.issn.1009-8291.2023.06.009
- VernacularTitle:组蛋白乙酰转移酶KAT7抑制剂WM-3835对膀胱癌细胞增殖和迁移的影响
- Author:
Wang WANG
1
;
Kangping XIONG
1
;
Kaiyu QIAN
1
,
2
;
Xinghuan WANG
1
Author Information
1. Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan 430071
2. Hubei Provincial Human Genetic Resources Conservation Center, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
- Publication Type:Journal Article
- Keywords:
bladder cancer;
WM-3835;
proliferation;
migration;
cell cycle;
chemotherapeutic agent
- From:
Journal of Modern Urology
2023;28(6):500-505
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the effects of WM-3835, a histone acetyltransferase KAT7 (KAT7) inhibitor, on the proliferation and migration of bladder cancer cells and to explore the possible mechanism. 【Methods】 Human ureteral epithelial immortalized cell line SV-HUC-1, and bladder cancer cell lines UM-UC-3 and T24 were treated with different concentrations of WM-3835 (0, 10, 20, 30, 40 μmol/L). After 48 hours, the effects of WM-3835 on the proliferation, cell cycle distribution and migration of cells were detected with MTT assay, flow cytometry, scratch and Transwell assay, respectively. The expressions of cyclin D1 (cyclin D1), proliferating nuclear antigen (PCNA), matrix metalloproteinase 9 (MMP9) and neurocadherin (N-cadherin) were detected with Western blotting and real-time quantitative PCR. 【Results】 WM-3835 significantly inhibited the proli-feration of bladder cancer cells in a dose-dependent manner. After treatment with WM-3835, the cycle of UM-UC-3 and T24 cells were blocked in the G0/G1 phase, the proliferation was effectively inhibited, and the migration was significantly wea-kened. The expressions of cyclin-D1, PCNA, MMP9 and N-cadherin were down-regulated. 【Conclusion】 WM-3835 can inhibit the proliferation and migration of bladder cancer cells, and has the potential as a chemotherapeutic agent for bladder cancer.
