Analysis of family mutation type and two-hit hypothesis of a familial VHL Syndrome patient
10.3969/j.issn.1009-8291.2023.09.014
- VernacularTitle:家族性VHL综合征患者家系突变类型及二次打击的分析
- Author:
Jun PAN
1
;
Yuxin CHEN
1
;
Yanwen LU
1
;
Xiang DONG
1
;
Haiyang YANG
2
;
Gutian ZHANG
1
;
Weidong GAN
1
;
Hongqian GUO
1
Author Information
1. Department of Urology, Affiliated Drum Tower Hospital of Medical College of Nanjing University, Nanjing 210008
2. Drum Tower Clinical Medical College of Jiangsu University, Zhenjiang 212000, China
- Publication Type:Journal Article
- Keywords:
renal cell carcinoma;
VHL;
syndrome;
familial;
database;
two-hit hypothesis
- From:
Journal of Modern Urology
2023;28(9):799-804
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To explore the mutation type, clinical characteristics, molecular genetics and the two-hit type of a patient with familial Von Hippel Lindau (VHL) syndrome. 【Methods】 The data of the patient were collected. DNA was extracted from the peripheral blood and renal cell carcinoma sample. The VHL gene germline mutation site was detected with high throughput sequencing next generation sequencing (NGS). The two-hit site was identified with UCSCXena database, methylation-specific PCR (MSP) and microsatellite stability detection. 【Results】 The mutation site of the embryo line was located in c.500G>A R167Q mutation. The patient had single nucleotide polymorphism, but no clear loss of heterozygosity, methylation or system mutation. 【Conclusion】 The germline mutation in exon 3 is the basis for the clinical features of this familial renal cell carcinoma proband. The identification of the two-hit site is key to the occurrence of the disease, which is significant for the diagnosis and treatment. The use of the databases can guide the screening of mutations and methylation sites in familial renal cell carcinoma.
