Identification and anti-infection effect of heparin binding motif of sacbrood virus capsid protein
10.13200/j.cnki.cjb.000077
- VernacularTitle:囊状幼虫病病毒衣壳蛋白肝素结合基序鉴定及其抗感染作用分析
- Author:
SHEN Kefei
- Publication Type:Journal Article
- From:
Chinese Journal of Biologicals
2023;36(12):1465-1470+1475
- CountryChina
- Language:Chinese
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Abstract:
Objective To identify the heparin-binding motif of drug-binding pocket protein 2(Dbpp2)of sacbrood virus(SBV)and determine the anti-SBV infection activity of the heparin-binding motif and its derived peptides.MethodsThe secondary structure prediction and homology modeling of Dbpp2 were carried out using online software to search for potential heparin-binding motifs. The binding ability of SBV,Dbpp2 and their heparin-binding motif mutants to and the ability of heparin-binding motifs to inhibit the binding of Dbpp2 to heparin were tested using heparin agarose beads. The therapeutic effect of heparin-binding motifs and their derived peptides on the larvae of Apis cerana cerana infected with SBV was tested.ResultsSBV and Dbpp2 showed binding with heparin. The flexible ring(KPANRPRR)rich in basic amino acids exposed at the C-end of Dbpp2 was a heparin-binding motif,which inhibited Dbpp2 from binding to heparin. KPANRPRR and its derived peptides KPAARPRR,KPRNRPRR,KPRARPRR,KPRWRPRR and KPRWRPRRW all had the effect of reducing the mortality of larvae caused by SBV infection,among which KPRARPRR showed the best therapeutic effect.Conclusion The research provides a basis for understanding the interaction of SBV-glycosaminoglycan(GAG),and provides a reference for the development of peptides for the treatment of SBV infection based on the interaction.
