Comparison of methods for detection of antibody level in serum immunized with SARS-CoV-2 mRNA vaccine
10.13200/j.cnki.cjb.000081
- VernacularTitle:SARS-CoV-2 mRNA疫苗免疫血清抗体水平检测方法的比较
- Author:
WU Xiaohong
- Publication Type:Journal Article
- Keywords:
Enzyme-linked immunosorbent assay(ELISA);
Pseudo virus-based neutralization assay(PBNA);
Micro-cytopathic effect neutralization test(MCPENT);
SARS-CoV-2;
mRNA vaccine
- From:
Chinese Journal of Biologicals
2023;36(12):1414-1418
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare three methods for detection of antibody level in serum immunized with SARS-CoV-2mRNA vaccine. Methods Enzyme-linked immunosorbent assay(ELISA),pseudo virus-based neutralization assay(PBNA)and micro-cytopathic effect neutralization test(MCPENT)were used to detect the antibody levels of a total of 120 serum samples(40 before immunization and 80 after immunization)before and after 2 doses of mRNA vaccine immunization,and the consistency and correlation of the three methods were analyzed. Results The consistency rates of the three methods detecting 120 serum samples were all over 90%,the Kappa coefficients were all more than 0. 7,and each P was less than0. 01. The correlation coefficient(r)between the antibody potency results of positive serum samples detected by the three methods was 0. 825～0. 902,and each P was less than 0. 01. Conclusion The three methods have good consistency and correlation in detecting antibody level of serum immunized with SARS-CoV-2 mRNA vaccine.
