Effects of total flavone of oldenlandia diffusa on the proliferation and apoptosis of hepatocellular carcinoma stem cell

Bowen Yao; Yazhao LI; Zijun Liao; Ye LU; Xiang Zhang; Jiequn MA; Qian LI; Yanbing Zhang

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Effects of total flavone of oldenlandia diffusa on the proliferation and apoptosis of hepatocellular carcinoma stem cell

VernacularTitle:白花蛇舌草总黄酮对肝细胞癌干细胞增殖及凋亡的影响
Author: Bowen YAO ¹ ; Yazhao LI ² ; Zijun LIAO ³ ; Ye LU ¹ ; Xiang ZHANG ⁴ ; Jiequn MA ³ ; Qian LI ³ ; Yanbing ZHANG ³
Author Information

1. Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061
2. Center for Translational Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061
3. Department of Oncology, Shaanxi Provincial Cancer Hospital, Xi’an 710061
4. Department of Cardiac Diagnosis, The Affiliated Children Hospital of Xi’an Jiaotong University, Xi’an 710003, China
Publication Type:Journal Article
Keywords: hepatocellular carcinoma; stem cell; total flavone of oldenlandia diffusa (FOD); proliferation; apoptosis
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2023;44(3):389-395
CountryChina
Language:Chinese
Abstract: 【Objective】 To investigate the effects of total flavone of oldenlandia diffusa (FOD) on the proliferation and apoptosis of hepatocellular carcinoma (HCC) stem cells sorted from Huh7. 【Methods】 Human HCC cell lines Huh7 was cultured in vitro; CD133 positive (CD133⁺) stem cells in Huh7 cell line were sorted by flow cytometry, and stem cell markers such as Nanog, Oct4 and Sox2 were tested by Western blotting. CD133⁺-Huh7 was stimulated by different concentrations (0 μg/mL, 50 μg/mL, 100 μg/mL and 400 μg/mL) of FOD for different time (24 h, 48 h, 72 h and 96 h). CCK8 and plate cell cloning assay were used to detect the effect of FOD on CD133⁺-Huh7 proliferation while Annexin V-PE/7-AAD was used to detect the effect of FOD on CD133⁺-Huh7 apoptosis. Western blotting was used to detect the protein expressions of protein 53 (P53), factor associated suicide-Fas-associating protein with a novel death domain (Fas-FADD), B-cell lymphoma-2 (Bcl-2), Cleaved-Caspase3, and Bcl-2 associated X protein (Bax). 【Results】 More than 95% of stem cells were purified for further experiments. Cell proliferation of CD133⁺-Huh7 was significantly inhibited by FOD, with the significant suppression at the concentration of 100 μg/mL for 72 h compared with negative control group (P<0.05). The apoptosis rate was significantly upregulated than that in the negative control group (P<0.05). The protein expression of Bcl2 decreased while Bax and Cleaved-Caspae3 increased via FAS/FADDD and P53 axis. 【Conclusion】 FOD can significantly inhibit the proliferation and promote the apoptosis of CD133⁺-Huh7.