开放科学(资源服务)标识码(OSID) Effect of Tanshinone ⅡA on abnormal integration of granule cells in the dentate gyrus of mice with chronic epilepsy

Xinli Xiao; Zitong Qin; Kaixiang Ren; Yiqiu Zheng; Hao Peng; Qiqi Qiang; Chutong Zhang; Zhuoyuan Yang; Qi Lei; Jianxin Liu

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开放科学(资源服务)标识码(OSID) Effect of Tanshinone ⅡA on abnormal integration of granule cells in the dentate gyrus of mice with chronic epilepsy

VernacularTitle:丹参酮ⅡA对癫痫慢性期小鼠齿状回颗粒细胞病理整合的影响
Author: Xinli XIAO ¹ ; Zitong QIN ² ; Kaixiang REN ² ; Yiqiu ZHENG ² ; Hao PENG ² ; Qiqi QIANG ² ; Chutong ZHANG ² ; Zhuoyuan YANG ³ ; Qi LEI ⁴ ; Jianxin LIU ⁵
Author Information

1. Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Xi’an Jiaotong University Health Science Center, Xi’an 710061
2. Clinical Medicine, Xi’an Jiaotong University Health Science Center, Xi’an 710061
3. Specialty of Anesthesiology, The Second Affiliated Hospital of Xi’an Medical College, Xi’an 710038
4. Department of Neurology, Shaanxi Provincial People’s Hospital, Xi’an 710068
5. Institute of Neurobiology, School of Basic Medical Sciences, Xi’an Jiaotong University Health Science Center, Xi’an 710061, China
Publication Type:Journal Article
Keywords: temporal lobe epilepsy; Tanshinone ⅡA; dentate gyrus; mTOR pathway
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2023;44(4):634-643
CountryChina
Language:Chinese
Abstract: 【Objective】 To clarify the role and molecular mechanism of Tanshinone ⅡA (TanⅡA) in the pathological integration of granule cells in the dentate gyrus (DG) by using the mouse model of temporal lobe epilepsy (TLE). 【Methods】 Status epilepticus (SE) was induced in the mice with pilocarpine and treated with TanⅡA 5 mg/kg. After two months, Morris water maze was used to examine the spatial learning and memory ability and video surveillance was used to monitor spontaneous seizures. The DG was removed for staining of Timm, Prox-1, DCX and SynⅠ. PTEN, p-AKT, and p-S6 expressions were observed by Western blotting. 【Results】 TanⅡA decreased Timm score, SynⅠ, PSD-95 and pS6 levels, and increased the level of PTEN in the DG, and attenuated the formation of mossy fiber sproutings and basal dendrites of the granule cells. Video surveillance showed that TanⅡA reduced the frequency of Racine’ grade 5 seizures. 【Conclusion】 TanⅡA can effectively attenuate the abnormal integration of the granule cells in the DG by regulating PTEN/AKT/mTOR pathway and thus plays an anti-epileptic role.