Effects of neuropathic pain induced by selective nerve injury model on intestinal microflora diversity in mice

Hui Wang; Yongxiao Cao; Huiyong Nie; Shuang LI; Gang BU

Return

Effects of neuropathic pain induced by selective nerve injury model on intestinal microflora diversity in mice

VernacularTitle:选择性神经损伤模型所致神经病理性疼痛对小鼠肠道菌群多样性的影响
Author: Hui WANG ¹ ; Yongxiao CAO ² ; Huiyong NIE ¹ ; Shuang LI ³ ; Gang BU ¹
Author Information

1. Department of Pain, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, China
2. Department of Pharmacology, Xi’an Jiaotong University Medical Science Center, Xi’an 710061, China
3. Department of Anesthesiology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, China
Publication Type:Journal Article
Keywords: neuropathic pain; intestinal flora; diversity; gut-brain axis
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2023;44(4):549-556
CountryChina
Language:Chinese
Abstract: 【Objective】 To investigate the effects of neuropathic pain induced by selective nerve injury (SNI) on intestinal microflora diversity in C57 mice. 【Methods】 36 C57 mice were randomly divided into SNI model group (n=18), sham-operation group (n=8), and control group (n=10). At day 0,1, 3, 7, and 14 after modeling, mechanical pain threshold and thermal pain sensitivity tests were carried out. At day 14 after modeling, colon content (fresh feces) from all the mice were collected for intestinal microflora diversity analysis. 【Results】 One day after modeling, the mechanical pain threshold in SNI group decreased significantly (more than 70%) due to nerve injury, and the thermal pain threshold decreased by 40%, while sham group and control group had no significant decrease. SNI group showed foot hyperalgesia, and the difference was statistically significant compared with sham group and control group (P<0.001). Compared with control group, sham-operation group had a transient decrease in thermal pain threshold on the first day after modeling (P=0.006), but there was no difference in pain threshold between the two groups on the third day after modeling. The α-diversity analysis showed that the abundance of Observed, Chao1, ACE and Simpson in SNI group was significantly lower than that in control group (P<0.05). That is, SNI group had flora disorder due to pain stimulation. Observed, Chao1, ACE, and Simpson were less abundant in sham group than in control group (P<0.05) and the change was between SNI group and control group. 【Conclusion】 Neuropathic pain induced by SNI model resulted in the decrease of mechanical pain threshold and thermal pain threshold, which leads to the reduction of intestinal flora diversity in C57 mice.