Biological characteristics of Escherichia coli phage and Staphylococcus aureus phage isolated from sewage

Xiangni Wang; Caiqin MA; Jinren Liu; Na Liu; Xiying Wang; Jiajia LU; Chuchu Kang; Xingmin Shi; Wang Yuan

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Biological characteristics of Escherichia coli phage and Staphylococcus aureus phage isolated from sewage

VernacularTitle:污水中分离的大肠杆菌噬菌体和金黄色葡萄球菌噬菌体的生物学特性分析
Author: Xiangni WANG ^{1
,

2} ; Caiqin MA ^{1
,

3} ; Jinren LIU ¹ ; Na LIU ¹ ; Xiying WANG ¹ ; Jiajia LU ¹ ; Chuchu KANG ¹ ; Xingmin SHI ¹ ; Wang YUAN ⁴
Author Information

1. School of Public Health, Xi’an Jiaotong University Health Science Center, Xi’an 710061
2. Yuncheng Vocational and Technical University, Yuncheng 044000
3. Medical Affairs Office, Second Affiliated Hospital, Airforce Military Medical University, Xi’an 710038, China
4. Department of PET-CT, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061
Publication Type:Journal Article
Keywords: Escherichia coli; Staphylococcus aureus; phage; biological characteristic; biological prevention and control
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2023;44(5):823-829
CountryChina
Language:Chinese
Abstract: 【Objective】 Escherichia coli phage (ECP) and Staphylococcus aureus phage (SAP) isolated from sewage were used as research objects, and their biological characteristics were analyzed to provide new experimental materials for the application of phages. 【Methods】 ECP and SAP were purified and cultured by double-layer agar method. Then a series of biological characteristics of these two phages were preliminarily analyzed by electron microscope observation, optimal multiplicity of infection (MOI) test, one-step growth curve test, temperature, pH, chloroform and ultraviolet sensitivity tests, respectively. 【Results】 The results of biological characteristics showed that ECP and SAP were both virulent phages, belonging to myoviridac family. Their optimal MOI was 10^-1, and they had strong resistance to ultraviolet light. The cleavage volume of ECP was 76.3 PFU/cell, while that of SAP was 8.3 PFU/cell. ECP had a wide range of temperature tolerance and could stably survive at 30-50 ℃, while SAP was more sensitive to temperature and could be completely inactivated at 50 ℃ for 1 h. ECP could maintain a good lysis activity in the range of pH 5-11, while SAP in the range of pH 6-9. ECP had strong resistance to chloroform and was non-membranous phage, while SAP was more sensitive to chloroform and was a membranous phage. 【Conclusion】 ECP and SAP are both virulent phages and have strong resistance to ultraviolet light. The lysability, temperature, pH, and chloroform tolerance of ECP are stronger than those of SAP.