Osteoclast-mediated pain and collapse mechanisms in the femoral head necrosis with bone marrow edema

Liang MO; Zhangzheng Wang; Chi Zhou; Zhenqiu Chen; Haibin Wang; Jiake XU; Yuhao Liu

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Osteoclast-mediated pain and collapse mechanisms in the femoral head necrosis with bone marrow edema

VernacularTitle:破骨细胞介导的股骨头坏死骨髓水肿疼痛及塌陷机制
Author: Liang MO ¹ ; Zhangzheng WANG ¹ ; Chi ZHOU ^{1
,

2} ; Zhenqiu CHEN ^{1
,

2} ; Haibin WANG ^{1
,

2} ; Jiake XU ³ ; Yuhao LIU ^{1
,

2}
Author Information

1. Guangzhou University of Chinese Medicine, Guangzhou 510006
2. The First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510405
3. Shenzhen Institute of Advanced Technology-Chinese Academic of Sciences, Shenzhen 518053, China
Publication Type:Journal Article
Keywords: osteonecrosis of femoral head; bone marrow edema; pain; collapse; osteoclast; netrin-1
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2023;44(6):950-957
CountryChina
Language:Chinese
Abstract: 【Objective】 To explore pain and collapse mechanisms in fosteonecrosis of the femoral head (ONFH) with bone marrow edema (BME). 【Methods】 ONFH patients at ARCO Ⅲ stage who underwent total hip arthroplasty in the First Affiliated Hospital of Guangzhou University of Chinese Medicine were enrolled; the femoral head samples, clinical and imaging data were collected. These patients were divided into BME group and non-BME group according to the MR data in one week preoperative. Hematoxylin-eosin and Sirius red staining were performed to observe the morphological changes in bone tissue of femoral head specimens. Western blotting and qPCR were used to semi-quantitatively analyze the expression levels of CTSK, RANKL, and Netrin-1 proteins and mRNA in different regions of the bone tissue. 【Results】 Clinical and imaging data showed that ONFH patients with BME had significantly higher scores of VAS than ONFH patients without BME. Hematoxylin-eosin staining showed that bone structure disorder and a large number of empty bone lacunae were found in the necrotic areas in both groups, but there exited significant granulation tissue in the BME group, and spindle-shaped fibroblastic cells and inflammatory cells aggregated in the repaired region. Sirius red staining revealed the necrotic and sclerotic areas were accumulated with many collagenous fiber in the BME group. The results of Western blotting and qPCR showed that Netrin-1 expressions in the necrotic, sclerotic and health areas in the BME group were higher than those in the non-BME group (P<0.05), while osteoclast related proteins and mRNA expressions of the necrotic and sclerotic areas in the BME group was higher than those in the non-BME group (P<0.05). 【Conclusion】 All these findings indicated that hip pain was positively correlated with femoral head necrosis with BME, hyperactive osteoclasts participated in the femoral head collapse with BME, and the upregulated expression of Netrin-1 mediated the pain mechanism.