Effect of total flavone of oldenlandia diffusa on the proliferation, apoptosis and stemness of breast cancer MDA-MB-231 cell lines

Bowen Yao; Yazhao LI; Jingyu LI; Chaoyi LI; Ye LU; Jiequn MA; Yanbing Zhang

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Effect of total flavone of oldenlandia diffusa on the proliferation, apoptosis and stemness of breast cancer MDA-MB-231 cell lines

VernacularTitle:白花蛇舌草总黄酮对乳腺癌MDA-MB-231细胞系增殖、凋亡及干性的影响
Author: Bowen YAO ¹ ; Yazhao LI ² ; Jingyu LI ³ ; Chaoyi LI ³ ; Ye LU ¹ ; Jiequn MA ⁴ ; Yanbing ZHANG ⁴
Author Information

1. Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061
2. Center for Tranlational Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061
3. Zonglian College, Xi’an Jiaotong University, Xi’an 710061
4. Department of Oncology, Shaanxi Provincial Cancer Hospital, Xi’an 710061, China
Publication Type:Journal Article
Keywords: breast cancer; stem cell; total flavone of oldenlandia diffusa (FOD); proliferation; apoptosis
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2023;44(6):880-885
CountryChina
Language:Chinese
Abstract: 【Objective】 To investigate the effect of total flavone of oldenlandia diffusa(FOD) on the stemness, proliferation and apoptosis of breast cancer(BC) stem cells sorted from MDA-MB-231. 【Methods】 Human BC cell lines MDA-MB-231 was cultured in vitro; MDA-MB-231 was stimulated by different concentrations(0 μg/mL, 100 μg/mL, 200 μg/mL and 400 μg/mL) of FOD for different time (24 h, 48 h and 72 h). CCK8 and plate cell cloning assay were used to detect the effect of FOD on MDA-MB-231 proliferation; CD44⁺/CD24^-MDA-MB-231 cell line were tested by flow cytometry and stem cell markers such as Nanog, Oct4 and Sox2 were tested by Western blotting; Annexin V-PE/7-AAD was used to detect the effect of FOD on MDA-MB-231 apoptosis and Bcl2, cleaved-caspase3 and Bax were tested by Western blotting. 【Results】 Cell proliferation of MDA-MB-231 was significantly inhibited by FOD, with the significant suppression at concentrations of 400 μg/mL for 72 h compared with negative control group(P<0.05). The apoptosis rate was significantly upregulated than the negative control group (P<0.05). The protein expression of Bcl2 decreased while Bax and cleaved-caspae3 increased, and stemness markers such as Nanog, Sox2 and Oct4 decreased in FOD-treated cells. Moverover, Akt-GSK3β-β-catenin axis was inhibited in FOD-treated cells. 【Conclusion】 FOD could significantly inhibit the stemness and proliferation and promote the apoptosis of MDA-MB-231.