Bone marrow mesenchymal stem cell-derived exosomal miR-30e-5p ameliorates high-glucose induced renal proximal tubular cell pyroptosis

Jia LÜ; Yaning Hao; Xiaopei Wang; Wanhong LU; Yali Zhang; Dan Niu

Return

Bone marrow mesenchymal stem cell-derived exosomal miR-30e-5p ameliorates high-glucose induced renal proximal tubular cell pyroptosis

VernacularTitle:骨髓间充质干细胞来源外泌体miR-30e-5p改善高血糖诱发的肾小管上皮细胞的焦亡
Author: Jia LÜ ¹ ; Yaning HAO ¹ ; Xiaopei WANG ¹ ; Wanhong LU ¹ ; Yali ZHANG ¹ ; Dan NIU ¹
Author Information

1. Department of Nephrology, Nephrotic Hospital, The First Affiliated Hospital of Xi’an Jiaotong University Health Science Center, Xi’an 710061, China
Publication Type:Journal Article
Keywords: pyroptosis; bone marrow mesenchymal stem cells; exosome; miR-30e-5p; diabetic kidney disease
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2023;44(6):859-865
CountryChina
Language:Chinese
Abstract: 【Objective】 To study the effects of miR-30e-5p from bone marrow mesenchymal stem cell-derived exosomes(BMSC-exos) on high glucose (HG)-induced HK-2 cell pyroptosis and explore an alternative strategy to manage diabetic kidney disease (DKD). 【Methods】 BMSC-exos were isolated and internalized into HK-2 cells treated with HG to measure viability and cytotoxicity. The secretion of IL-1β and IL-18 was measured by ELISA. Pyroptosis was assessed by flow cytometry. The levels of miR-30e-5p, IL-1β, and IL-18 were measured. The expression of pyroptosis-associated cytokine proteins was determined. 【Results】 BMSC-exos decreased LDH, IL-1β, and IL-18 secretion and inhibited the expression of the pyroptosis-related factors (IL-1β, caspase-1, GSDMD-N, and NLRP3) in HG-induced HK-2 cells. Moreover, miR-30e-5p depletion in BMSC-exos promoted HK-2 cell pyroptosis. 【Conclusion】 BMSC-derived exosomal miR-30e-5p inhibits caspase-1-mediated pyroptosis in HG-induced HK-2 cells, which might provide a new strategy for treating DKD.