Isoliquiritigenin suppresses the inflammatory response of vascular endothelial cells via HDAC3

Zhiyan LU; Yinan LI; Yue Yuan; Ziyang MA; Yuanlin Luo; Lifang Chen; Yirong Zhang; Weirong Wang

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Isoliquiritigenin suppresses the inflammatory response of vascular endothelial cells via HDAC3

VernacularTitle:异甘草素通过HDAC3抑制血管内皮细胞的炎症反应
Author: Zhiyan LU ^{1
,

2} ; Yinan LI ^{1
,

2} ; Yue YUAN ^{1
,

2} ; Ziyang MA ^{1
,

2} ; Yuanlin LUO ^{1
,

2} ; Lifang CHEN ^{1
,

2} ; Yirong ZHANG ^{1
,

2} ; Weirong WANG ^{1
,

2}
Author Information

1. Department of Laboratory Animal Science, School of Basic Medical Science of Xi’an Jiaotong University Health Science Center, Xi’an 710061
2. Institute of Cardiovascular Science, Translational Medicine Institute, Xi’an Jiaotong University Health Science Center, Xi’an 710061, China
Publication Type:Journal Article
Keywords: isoliquiritigenin; HDAC3; vascular endothelial cell; inflammatory response
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2023;44(6):852-858
CountryChina
Language:Chinese
Abstract: 【Objective】 To investigate the effect of isoliquiritigenin on inflammatory response of vascular endothelial cells and whether the regulatory effect of isoliquiritigenin on inflammation is mediated by histone deacetylase 3 (HDAC3). 【Methods】 Human umbilical vein endothelial cells (HUVECs) were cultured in vitro and treated with LPS, different concentrations of isoliquiritigenin and HDAC3 specific inhibitor, respectively. Real-time PCR and Western blotting were used to detect the mRNA and protein expressions of inflammatory cytokines and HDAC3. Male C57BL/6J mice were randomly divided into vehicle group and isoliquiritigenin treatment group. The vascular inflammation model of C57BL/6J mice was established by ligation of the left carotid arteries. The mRNA expressions of inflammatory cytokines and HDAC3 in the carotid arteries of mice were detected by Real-time PCR. A molecular docking study was performed to investigate the interaction between isoliquiritigenin and HDAC3. 【Results】 Compared with the vehicle group, isoliquiritigenin reduced the mRNA expressions of inflammatory cytokines NLRP3, IL-1β, IL-18, MCP-1 and ICAM-1 and decreased the expression of HDAC3 mRNA and protein in HUVECs stimulated with LPS. In addition, isoliquiritigenin also decreased the mRNA expressions of NLRP3, IL-1β and HDAC3 in carotid arteries of ligated C57BL/6J mice. The docking of isoliquiritigenin in the active site of HDAC3 showed that isoliquiritigenin might act through HDAC3. Furthermore, HDAC3 specific inhibitor RGFP966 further promoted the inhibitory effect of isoliquiritigenin on the expression of inflammatory cytokines in vascular endothelial cells. 【Conclusion】 These results suggest that isoliquiritigenin suppresses the inflammatory response of vascular endothelial cells via HDAC3.