Puerarin reduces intracellular Ca2+ concentration and upregulates BDNF to protect hippocampal neurons in vascular dementia rats
- VernacularTitle:葛根素降低细胞内Ca2+浓度并上调BDNF保护血管性痴呆大鼠海马神经细胞
- Author:
Hongwei REN
1
;
Ming CHEN
1
;
Huqing WANG
1
;
Zhen GAO
1
;
Haiqin WU
1
Author Information
- Publication Type:Journal Article
- Keywords: puerarin; vascular dementia; Ca2+ concentration; brain-derived neurotrophic factor; neuroprotection
- From: Journal of Xi'an Jiaotong University(Medical Sciences) 2023;44(2):202-207
- CountryChina
- Language:Chinese
- Abstract: 【Objective】 To observe the effect of puerarin on the concentration of Ca2+ and the expression of brain derived neurotrophic factor (BDNF) in hippocampal neurons of vascular dementia (VD) rats so as to explore the mechanism of puerarin in protecting nerve cells. 【Methods】 Male SD rats were randomly divided into sham operation group, model group, and puerarin intervention group. The vascular dementia model was established by ligating bilateral common carotid arteries at intervals of 3 days. Two weeks after the operation, the learning and memory abilities of the rats were evaluated by Morris water maze, and the expression of BDNF in the hippocampus of the rats was detected by immunohistochemistry and Western blotting. The mean fluorescence intensity was measured by flow cytometry to represent the intracellular free Ca2+ concentration. 【Results】 In the puerarin intervention group, the rats’ escape latency in Morris water maze was significantly shortened, the expression of BDNF in the hippocampus was significantly increased, and the concentration of Ca2+ in hippocampal neurons was decreased. Compared with the model group, the difference was statistically significant (all P<0.05). 【Conclusion】 Puerarin has neuroprotective effect on VD rats, and its mechanism may be related to the decrease of Ca2+ concentration in hippocampal neurons and the up-regulation of BDNF expression.
