Effects of Hepatocyte Growth Factor on Collagen Synthesis and Matrix Metalloproteinase Production in Keloids.
10.3346/jkms.2011.26.8.1081
- Author:
Won Jai LEE
1
;
Sang Eun PARK
;
Dong Kyun RAH
Author Information
1. Institute for Human Tissue Restoration, Department of Plastic and Reconstructive Surgery, Yonsei University Health System, Severance Hospital, Seoul, Korea. pswjlee@yuhs.ac
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Hepatocyte Growth Factor;
Keloid Fibroblast;
Dermal Fibroblast;
Collagen;
Matrix Metalloproteinase
- MeSH:
Cells, Cultured;
Collagen Type I/genetics/metabolism;
Collagen Type III/genetics/metabolism;
Fibroblasts/drug effects/metabolism;
Hepatocyte Growth Factor/*pharmacology;
Humans;
Keloid/*metabolism/pathology;
Matrix Metalloproteinase 1/genetics/metabolism;
Matrix Metalloproteinase 2/metabolism;
Matrix Metalloproteinase 3/genetics/metabolism;
Matrix Metalloproteinase 9/metabolism;
RNA, Messenger/metabolism;
Transforming Growth Factor beta1/pharmacology
- From:Journal of Korean Medical Science
2011;26(8):1081-1086
- CountryRepublic of Korea
- Language:English
-
Abstract:
Keloids are pathologic proliferations of the dermal layer of the skin resulting from excessive collagen production and deposition. Hepatocyte growth factor (HGF) increases the expression of matrix metalloproteinase (MMP)-1 and suppresses collagen synthesis to modulate extracellular matrix turnover. To investigate the anti-fibrotic effects of HGF, we examine the mRNA expression of collagen types I and III and matrix metalloproteinase (MMP-1, MMP-3) on human dermal fibroblast (HDF) cell lines and keloid fibroblasts (KFs, n = 5) after adding various amount of HGF protein. We also evaluated the enzymatic activity of MMP-2, MMP-9 by zymograghy. In HDFs treated with TGF-beta1 and HGF protein simultaneously, both type I and III collagen mRNA expression significantly decreased (P < 0.05). Expression of MMP-1, MMP-3 mRNA also decreased. However, the mRNA expression of MMP-1, MMP-3 significantly increased in KFs with increasing amount of HGF in dose dependent manner (P < 0.05). The enzymatic activities of MMP-2 increased with increasing HGF protein in a dose-dependent manner. However, the enzymatic activity of MMP-9 did not change. These results suggest that the anti-fibrotic effects of HGF may have therapeutic effects on keloids by reversing pathologic fibrosis.
