Preparation and in vitro evaluation of polylactic acid nanoparticles containing arginine and glucose oxidase
10.16438/j.0513-4870.2023-0293
- VernacularTitle:载精氨酸和葡萄糖氧化酶的聚乳酸纳米粒子制备与体外评价
- Author:
Mei-yang YANG
1
,
2
;
Wei-jun CHEN
1
;
Li-peng QIU
1
;
Jing-hua CHEN
1
Author Information
1. School of Life Science and Health Engineering, Jiangnan University, Wuxi 214122, China
2. School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China
- Publication Type:Research Article
- Keywords:
italic>L-arginine;
glucose oxidase;
polylactic acid;
hydrogen peroxide;
nitric oxide
- From:
Acta Pharmaceutica Sinica
2024;59(1):225-231
- CountryChina
- Language:Chinese
-
Abstract:
Hydrogen peroxide (H2O2) and nitric oxide (NO) has a short half-life, low bioavailability, poor tumor targeting and systemic adverse reactions in the physiological environment. In this study, phacoemulsification and nano-precipitation were used to synthesize didecyl dimethyl ammonium bromide (DDAB)/polylactic acid nanoparticles (PLA), then L-arginine (L-Arg) and glucose oxidase (GOx)-loaded nanoparticles (GADP) were prepared, and the in vitro antitumor activity was investigated．The particle size, potential, embedding rate and the ability to produce H2O2/NO of the nanoparticles were investigated. Meanwhile, in vitro cell cytotoxicity against human hepatoma cells (HepG2) was evaluated．The results showed that the prepared L-Arg-DDAB/PLA (ADP) nanoparticles were spherical particles. And the particle size and zeta potential were (225.7 ± 6.33) nm and (+23.5 ± 0.12) mV, respectively. The adsorption rate of GOx was 87.23% ± 0.02%. The drug loading of L-Arg was 15.6% ± 0.22%. The pH value of glucose solution and the amount of H2O2 showed that GADP had good catalytic activity. In vitro cytotoxicity experiments showed that blank nanoparticles were nontoxic, while the drug-loaded nanoparticles presented enhanced antitumor effect on HepG2 cells. And can inhibit tumor cell migration. The low dose nano-scale NO delivery system GADP can effectively inhibit the migration of tumor cells and kill tumor cells, thus producing therapeutic benefits.
