Mechanism of Qizhenziyin mixture in the treatment of hypogonadism based on network pharmacology analysis and molecular docking
10.12206/j.issn.2097-2024.202208111
- VernacularTitle:基于网络药理学和分子对接技术探讨杞贞滋阴合剂治疗性腺功能减退症的作用机制
- Author:
Yujiong PAN
1
;
Zhigao HE
1
;
Shixiu CHEN
1
;
Gui ZHOU
1
;
Xin ZHOU
1
;
Chao YU
1
Author Information
1. Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
- Keywords:
Qizhenziyin mixture;
hypogonadism;
network pharmacology;
molecular docking
- From:
Journal of Pharmaceutical Practice and Service
2024;42(1):24-31
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of Qizhenziyin mixture in the treatment of hypogonadism by using the network pharmacology approach. Methods The active components of Qizhenziyin mixture were obtained by searching TCMSP ，TCMID and HIT databases.The related targets of candidate compounds were obtained by searching STITCH databases. The potential targets of Qizhenziyin mixture in the treatment of hypogonadism were obtained by mapping the disease genes of hypogonadism with Genecards and DisGeNet databases. The protein interaction platform database (STRING) was used to construct the interaction relationship between action targets. The target protein interaction （PPI） network was constructed by introducing Cytoscape software. The mechanism of Qizhenziyin mixture in the treatment of hypogonadism was explained through the enrichment analysis of GO, KEGG and molecular docking technology. Results A total of 148 drug-disease chemical compounds, 96 drug-disease intersection targets, 1085 disease targets were obtained；the components for treating diseases are: quercetin，kaempferol, luteolin, etc; enrichment analysis of GO revealed 1792 biological processes （BP）, 31 cellular components (CC) and 79 molecular functions (MF)；the results of KEGG pathway enrichment analysis indicated such as FOXO signaling pathway, prostate cancer, AGE-RAGE signaling pathway in diabetic complications, HIF-1 signaling pathway, etc.The results of molecular docking showed that kaempferol and LEP had the best and stable binding energy. Conclusion The active components of Qizhenziyin mixture may play a role of the treatment of hypogonadism by improving insulin resistance and the expression of testosterone synthetase of Leydig cells.
