Effect of zinc and probiotics supplementation on IL-6 and tissue neutrophil levels in rats exposed to cigarette smoke
https://doi.org/10.31246/mjn-2022-0116
- Author:
Putu Gita Andryani
1
;
Endang Mahati
2
;
Kusmiyati Tjahjono
3
;
Yan Wisnu Prajoko
4
;
Neni Susilaningsih
5
;
Hermawan Istiadi
6
Author Information
1. Biomedical Science, Faculty of Medicine, Diponegoro University, Semarang, Central Java, Indonesia
2. Department of Pharmacology and Therapeutics, Faculty of Medicine, Diponegoro University, Semarang, Central Java, Indonesia
3. Department of Biochemistry, Faculty of Medicine, Diponegoro University, Semarang, Central Java, Indonesia
4. Department of Surgery, Faculty of Medicine, Diponegoro University, Semarang, Central Java, Indonesia
5. Department of Histology, Faculty of Medicine, Diponegoro University, Semarang, Central Java, Indonesia
6. Department of Anatomical Pathology, Faculty of Medicine, Diponegoro University, Semarang, Central Java, Indonesia
- Publication Type:Journal Article
- Keywords:
cigarette smoke, IL-6, neutrophils, probiotics, zinc
- From:
Malaysian Journal of Nutrition
2023;29(No.2):333-342
- CountryMalaysia
- Language:English
-
Abstract:
Introduction: Cigarette smoke exposure can cause inflammation, inducing the release of acute phase cytokines, such as IL-6, that will then trigger the recruitment of neutrophils, which are mostly phagocytic cells. Zinc and probiotics are known to have beneficial effects against inflammation. This study was conducted to investigate the effect of zinc and probiotics supplementation on IL-6 and tissue neutrophil levels in rats exposed to cigarette smoke. Methods: In a randomised, experimental study
with post-test control group design, thirty 2 to 3-month-old male Wistar rats, each weighing 180-220 g, were divided into five groups: control group without treatment (C); exposed to cigarette smoke [C (-)]; exposed to cigarette smoke and received zinc (Z); exposed to cigarette smoke and received probiotics (P); and exposed to cigarette smoke and received a combination of zinc and probiotics (ZP). Results:
Mean tissue neutrophil levels in Z, P, and ZP groups were 43.43±2.01, 34.67±1.32,and 29.77±5.05 cells, respectively. There were significant differences between supplementation intake and tissue neutrophil levels in each group compared to C (-) group (p<0.05). Meanwhile, only IL-6 level in the ZP group (6.02 pg/mL) decreased significantly compared to C (-) group (10.61 pg/mL). Conclusion: These results suggest that a combination of zinc and probiotics have an anti-inflammatory effect as measured by IL-6 and neutrophil levels.
- Full text:4.2023my0620.pdf
